DNA is life!
These bonds join the bases of complimentary DNA strands?
hydrogen
I unwinds DNA
Helicase, replication
What is supercoiling, which enzyme removes it, and which process is it found it
formation of knots when unwinding DNA, topoisomerase, replicaiton
This mutation causes no change to the amino acid sequence
silent
This allows proteins to be sorted to the rough ER
Bonus 100pts what binds to the ER sequence to help translocate the mRNA and ribosome to the the rough ER?
ER signaling sequence
Bonus signal recognition particle—SRP
A nucleotide is composed of
A phosphate group, a sugar and a nitrogenous base
I can start a RNA chain from scratch
Primase, replication
List the following enzymes in order from start (1) to finish (6)
-DNA ligase, DNA polymerase III, Helicase, SSB, DNA polymerase I, Primase
1. Helicase
2. SSB
3. Primase
4. DNA poly III
5. DNA poly I
6. DNA ligase
This mutation causes a premature stop codon
Nonsense
Name 2 posttransnational modifications that happen in the ER
Bonus 300pts give a function for each
Proteolytic cleavage
Glycosylation
Phosphorylation
This strand can only replicate in short fragments
Lagging strand
I add RNA nucleotides to a growing strand
RNA polymerase II, transcription
These things are needed to start transcription. All components must be listed!
a promoter with TATA box, transcription factors and RNA polymerase II
If 2 nucleotides are added to a sequence this mutation will occur
frameshift
This molecule is added to a protein if it is missfolded.
Bonus 100pts where does this happen?
ubiquitin
Bonus-ER
The complementary nucleotides to the following sequence:
ATTAGCTACTT
TAATCGATGAA
I remove the wrong bases during mismatch repair
DNA polymerase, DNA repair
To start translation these 5 things are required
ribosome, mRNA, tRNA, start codon, and initiation factors
This enzyme can remove incorrect bases pairs in the 3'-5 direction when proof reading
DNA polymerase III
This sequence is required for a transmemembrane protein
Bonus 100pts what does this sequence consist of?
Stop-transfer sequence
~20 hydrophobic amino acids that form a alpha helix in the membrane
In a growing polynucleotide the new nucleotide will be added on to the sugar's______carbon
3'
I am resspoisilbe for "charging" tRNAs
aminoacyl-tRNA synthetase, translation
Transcription takes place in the _____, while translation of proteins bound for the EMS system starts here_____and then are translocated to the_______.
Nucleus, cytoplasm, rough ER
UV radiation can cause this type of mutation and it can be repair by this process
pyrimidine dimers, nucleotide excision repair
The pathway a protein takes to be secreted from a cells
Bonus 100pts which side of the Golgi does a protein enter?
rough ER, vesicle, Golgi, vesicle, plasma membrane