DNA Info/Structure
DNA Replication
Mutations
Mitosis/Meiosis
MISC
100
What direction is DNA read?
What direction is the mRNA made?
DNA read - 5' to 3'
mRNA made - 5' to 3'
100
Draw/Label a Replication Bubble: Leading Strand, Lagging Strand, Okazaki Fragments, Replication Fork.
100
Sickle cell disease is caused by what type of mutation?
Missense mutation resulting from a substitution
100
What is the difference between Metaphase I and Metaphase II in Meiosis?
(Can draw the two phases)
The major difference is that in metaphase I, they are pairs of homologous chromosomes that line up, and in metaphase II, single chromosomes, which are not paired, line up at the midline of the cell.
100
How many bones are in the human body?
206
200
What are the 2 types of bonds in DNA and where are they located?
Hydrogen Bonds - Between Bases
Phosphodiester Bonds - Between Nucleotides
200
Which strand grows continuously towards the replication fork?
Leading Strand
200
Identify the type of mutation.
Original DNA Sequence: CTC/GCA/AGA/TTT
Copied DNA Sequence: CTC/GCA/ACG/ATT/T
Addition
200
What is the function of mitosis in Unicellular and Multicellular organisms?
What is the function of meiosis?
Unicellular - Asexual reproduction
Multicellular - Tissue renewal & regeneration
Growth & Development
Meiosis - Serves as reproduction in multicellular organisms
200
Where is the Great Barrier Reef located?
Australia
300
What are the 4 nucleotide bases in DNA and RNA?
DNA - Adenine, Guanine, Thymine, Cytosine
RNA - Adenine, Guanine, Uracil, Cytosine
300
What is the function of Topoisomerase?
Uncoil in front of the replication fork. Break one of the strands and ligate it back together.
300
The original amino acid sequence is Met-Arg-Glu. If a mutation occurs, and the mutated sequence is Met-Arg-Glu, what type of mutation occured?
Silent
300
Identify which are homologous pairs and which are sister chromatids.
SI Leader will draw on the board.
Homologous pairs - Red and Blue
Sister Chromatids - Either red OR blue side
300
What is the largest organ in the human body?
Skin
400
What are the levels of complexity for the following: chromosome, nucleotide, gene, DNA, genome.
nucleotide -> DNA -> gene -> chromosome -> genome
400
During mRNA processing in eukaryotes, splicing removes _____ from the mRNA transcript and keeps _____ in the mRNA transcript.
Removes INTRONS
Keeps EXONS
400
Assume each of the following letters represents an amino acid. What type of gene mutation occured to produce the following protein sequence?
Normal: JAYBIRDCATPAW
Mutated: JAYBIRDBATPAW
Missense
400
During anaphase I of meiosis, ____ move toward opposite cell poles, whereas during anaphase II of meiosis, _____ are seperated.
Homologous Chromosomes
Sister Chromatids
400
Who was the first Disney princess?
Snow White
500
List the order of proteins that are involved in DNA Replication
1. Helicase
2. SSBP
3. RNA Primase
4. DNA Polymerase removing RNA Nucleotides and replacing them with DNA Nucleotides
5. DNA Ligase
500
Which sequence of events correctly describes the initiation and elongation steps of translation in prokaryotic cells?
1. initiator tRNA binds start codon on mRNA
2. small ribosomal subunit binds to mRNA
3. large ribosomal subunit binds
4. tRNA entry and peptidyl transfer reaction
5. translocation of ribosome and release of tRNA
2, 1, 3, 4, 5
500
Identify the DNA and Protein Mutation for the following:
Original - 5' ATCGTATAATCTAGATATGGGACCTCCGGAGCAGCTCTGATCTTC 3'
3' TAGCATATTAGATCTATACCCTGGAGGCCTCGTCGAGACTCGAAG 5'
Original Polypeptide - N-MET-GLY-PRO-PRO-GLU-GLN-LEU-STOP-C
Mutant - 5' ATCGTATAATCTAGATATGGGACTCCGGAGCAGCTCTGAGCTTC 3'
3' TAGCATATTAGATCTATACCCTGAGGCCTCGTCGAGACTCGAAG 5'
Mutant Polypeptide - N-MET-GLY-LEU-ARG-SER-SER-PHE-GLU-LEU-C
DNA Mutation - Frameshift Deletion
Protein Mutation - Excessive Missense
500
Place the following events of mitosis in the correct order:
1. sister chromatids move to opposite poles
2. sister chromatids move to metaphase plate
3. the nuclear membrane breaks up
4. sister chromatids condense
5. microtubules attach to centromere of sister chromatids
4, 3, 5, 2, 1
500
Lake Superior State University offers questing licenses for what mythical animal?
A Unicorn