DNA Structure & Replication; RNA
The Cell Cycle
Protein Synthesis
Codon Practice
Biotechnology
100
What are the pairing rules for DNA? (50 pts)
What are the pairing rules for RNA? (50 pts)
DNA: A-T; C-G
RNA: A-U; C-G
100
List all 3 stages of the cell cycle
Interphase, mitosis (M-phase), cytokinesis (C-phase)
100
What is the central dogma of biology?
DNA makes RNA, RNA makes proteins
100
Find the amino acid for the following codon: ACC
Thr (Threonine)
100
Identify the volume amount for the following P20 micropipette (include the unit in your answer):
1
3
7
13.7 microliters
200
On which strand would you find an Okazaki fragment?
The lagging strand
200
List the steps of mitosis
Prophase, metaphase, anaphase, telophase (PMAT)
200
List the 3 steps of building a polypeptide
Initiation, elongation, termination
200
Find the amino acid for the following codon:GGG
Gly (Glycine)
200
Which enzyme did we use in Lab 3 that created our target plasmid, PARA-R?
DNA Ligase
300
List all 3 ways that DNA and RNA differ
(100 pts each)
1. STRANDS - DNA: double; RNA: single
2. SUGARS - DNA: deoxyribose; RNA: ribose
3. N-BASES - DNA: thymine; RNA: uracil
300
What are cells doing when they're in G0 phase?
Cells are doing their jobs
300
What is the difference between transcription and translation?
Transcription is making a copy of DNA; translation is creating proteins from mRNA
300
Find the codon for the following amino acid: MET (methionine)
AUG
300
Which fragment is the shortest? How do you know?
Fragment E, because it traveled the farthest through the gel.
400
What are the 4 enzymes needed for DNA replication?
Helicase, Primase, DNA Polymerase, Ligase
400
During which checkpoint do we see the following:
checking to see if DNA replication is complete; the DNA has no errors.
G2/M checkpoint
400
What's the difference between introns and exons?
introns are not needed (IN between); exons are important to the gene (EXpressed)
400
Find the codon for the following amino acid: TRP (tryptophan)
UGG
400
Which restriction enzyme below produces a blunt cut?
Hae III
500
In which direction is a new DNA strand built?
5' to 3' direction
500
What are the 2 things during the cell cycle that cause cancer?
1. loose checkpoints
2. accelerated rate of cell division
500
What is the purpose of mRNA?
It is a disposable copy of a gene
500
Find an amino acid with at least 6 codon redundancies
SER (serine), LEU (leucine), ARG (arginine)
500
Assuming all parts of our labs had no issues, would the bactera transformed with PARA-R show the rfp protein on the LB/AMP plate? Why or why not?
No, because rfp needs to be activated by the arabinose (ARA) sugar.