GMP Process
This size bioreactor production run typically does not have a feed up process.
What is a 250L scale?
This is the central dogma.
What is DNA - RNA - Protein?
This delivery method uses a vector/modified virus to deliver genes directly to patient cells.
What is In Vivo gene therapy?
This is how many attempts it took Alex van der Eb and Frank Graham to successfully immortalize the cell line we use today.
What is 293 times?
This is the operating temperature of the Metaflex.
What is 15-32C?
This parameter on all bioreactors maintains pressure and inflation at the top of the biocontainer and offers minor gas exchanges at the top of the culture.
What is Gas Overlay?
These enzymes are designed for each plasmid to cut DNA into fragments, creating "sticky ends"
What are restriction enzymes?
BONUS (300 points) - name a commonly used restriction enzyme.
This function of AAV gene therapy results in the desired genetic material being released into the host cell's cytoplasm.
What is transduction?
What is Lactate?
These are the three pumps we use in upstream production.
What are Masterflex L/S, Masterflex I/P, and Watson Marlow 530?
These are the two most common filter types that are used for Millipore pod holder for depth filtration.
What are D0SP and C0SP?
During protein synthesis, this region of DNA sequence is translated into a protein; defined by a start and stop codon.
What is an open reading frame (ORF)?
These is one of the most common diseases clients develop treatments for.
What is Duchenne Muscular Dystrophy (DMD)?
**DAILY DOUBLE**
This amount of cell culture suspension is needed to passage a 2.8L flask on a Friday if your live counts were 3.87e6, 4.03e6, and 4.12e6.
What is 125mL of cell culture?
These are the four analytes that we record for QC calibrations on the Metaflex.
What are pH, PCO2, cGlu, and cLac?
These fossilized remains are commonly used in clarification filters.
What is Diatomaceous Earth?
These short, single stranded (ss) nucleic acid sequences initiate DNA synthesis.
What are DNA primers?
This is considered the typical maximum size for AAV packaging.
What is 4.7kb (kilobase)
This is the standard calculated time in which cells reproduce within a 24hr period.
What is doubling time?
This is the equivalent tubing size setting on Masterflex L/S pump for a 3/8 x 5/8" tube.
What is L/S 36?
During inoculation and depth filtration this pump setting allows for tightening/loosening of the pump head on the tubing in order to increase/decrease the flow efficiency.
What is the occlusion setting?
Bonus to know what the occlusion setting should be prior to determining if adjustments are needed (200 points)
These are the three major metabolic pathways in energy metabolism in cells.
What are immunogenicity, potency/efficacy, and genotoxicity?
These are the target VCDs for both a 2-day carry and a 3-day carry.
What is 1.0e6 cells/mL (2 day) and 0.5 cells/mL (3 day)?
This is the maximum VCD the NC202 can read without performing a dilution.
What is 1e7 (1x10^7) cells/mL?