The Cell kitchen
(Media)
(Media)
What is the primary sugar source that cells consume for energy?
Glucose
Since CHO cells are mammalian cells, what temperature (°C) do they adapt at best?
37°C
If fuzzy green mold or culture turbidity were observed, what is this called?
Contamination
Besides the nutrient rich media and feed, what gas must be constantly introduced for cells metabolism?
Oxygen
What's the optimum pH for enhanced CHO cell growth?
7.00 +- 0.3
If cells are multiplying rapidly, will the DO levels in the tank go Up or Down?
Down as cells consume
What is the media mainly composed of ?
Aminoacids, pH buffer, vitamins, salts and growth factors.
Why do we gently spin the impeller inside the bioreactor tank?
for equal gas distribution and nutrient homogeneity
If a tank starts overflowing with bubbles and soap-like suds, what's the issue?
Foaming
Cell as they metabolize produce toxic by-products, such as....
Lactate and ammonia
What is the giant, shiny stainless steel tank used for cells' cultivation?
Stirred Tank Bioreactor
If the impeller spins fast creating a harsh environment for the cells, what is the engineering term for this physical force?
Shear Stress
Before putting the liquid media into the clean bioreactor, it must pass through a step called?
Sterile filtration
To grow enough cells to fill a massive 2000L manufacturing tank, you can't start there. what is a step-by-step process called where you move cells from a tiny vial to larger and larger flasks?
Seed train and Scaling Up
If the DO sensor shows 0%, but the cells are still alive and the air is pumping perfectly, what part of the equipment is likely broken?
The DO Sensor/Probe