How/Why It’s Used
What was the main scientific reason(s) Wide-field Microscopy was developed?
To provide rapid imaging of moving specimens.
What is the best type of specimens for Wide-field Microscopy to be used on?
A small or thin specimen works the best.
Can Wide-field Microscopy be used on the same sample over a long period of time?
Yes, and it commonly is due to its low photo-toxicity. (For the record though, it does still have limits to how long it can go on for.)
What was a financial reason for the development of Wide-field microscopy?
Wide-field Microscopy is cost effective and easy to maintain.
What is the worst type of specimen for Wide-field Microscopy to be used on?
A larger specimen with a thicker outside. In general, anything that easily scatters light.
Does the imaging from Wide-field Microscopy always come back clear?
It rarely does.
Does Wide-field Microscopy present a method of imaging with HIGH or LOW photo-toxicity
Wide-field Microscopy presents an imaging method with LOW photo-toxicity.
Does Wide-field Microscopy still work if a specimen is moving around rapidly?
Yes, Wide-field Microscopy is still able to capture a specimen even if it is moving due to its speed.
Would Wide-field Microscopy work on the E.coli used in lab?
Yes, especially if it is a thin layer of E.coli.
What type of light source is best for Wide-field Microscopy?
A broad-specimen light source like Mercury, LED, or Xenon Lamps.
In the study described, what was Wide-field Microscopy used for? (In general)
It was used to visualize a C. albicans invasion.
What about the setup of Wide-field Microscopy would need to be changed depending on the fluorophores present?
The excitation/emission filters and/or the light source can be changed to better suit the fluorophores present.
Briefly describe the process of Wide-field Microscopy. (Shorthand terms are fine)
Basically, light is filtered in, excites fluorophores, relaxed fluorophores emit a longer wavelength emission light, and that light is then observed.
In the study described, was Wide-field microscopy limited? If so, how?
There were limits as it was unable to efficiently visualize nano-scale structure due to resolution
In the event there are issues with the resolution or specimen when using Wide-field Microscopy, what is another method or work around that can be employed?
Confocal Microscopy can be used if the sample is too thick. Electron Microscope can be used to more precisely image smaller structures. Image editing software can be employed to clear up the resolution of Wide-field Microscopy images.