Vocab
Differences between individuals and the size of DNA fragments, generated by cutting them with RE's
What are Restriction Fragment Length Polymorphism (RFLP's)?
A huge collection of cloned DNA fragments
The process of focusing recombinant genetic material into a variety of micro-species
What is CRAGE (Chassis-independent recominase-assisted genome engineering)?
*Forces species to express thing they would normally only show under extreem stress*
New DNA is introduced into a cell (or when it gains new genetic abilities)
What is a transformation?
___ is created by cloning a huge number of random pieces of cut DNA
What is a genomic library?
*enough to show the whole genome*
Two problems with whole organism cloning
What are:
1. Lower survival rate
2. Epigentic change
___ has the ability to make a DNA strand from an RNA template
What is a Reverse Transcriptase?
Collections of tissue-specific genes (copies come from Reverse Transcriptase)
What is a cDNA Library?
Small, noncoding, regulatory sequences of RNA
What are microRNA's?
*reduce stability of strand*
Collections of all RNA or mRNA expressed by an individual
What is a Transcriptome Library?
Copies of messenger RNA transcripts (represent the nucleotides that would be present in a mature RNA)
What is Complementary RNA (or cRNA)?
The 3 step processing of producing large amounts of DNA from small samples
Steps:
1. Denaturation of original DNA
2. Annealing
3. Elongation