DNA segments on pGLO
Origin of plasmid replication
What is Ori?
The operon will not code for GFP.
What happens when arabinose is absent?
This plasmid tube contains pGLO.
What is the positive plasmid tube?
Segment of DNA that codes for the glowing gene.
What is GFP (Green Flourescent Protein)?
Arabinose is present.
When will transcription of GFP occur?
The bacteria on this plate will not survive the lab.
What is the negative LB/amp plate?
Genes that code for an enzyme that breaks down the antibiotic Ampicillin.
What is Bla?
Acts as a checkpoint that controls access to GFP.
What is the purpose of the Operator?
This process makes the cell membrane permeable.
What is heat shock?
Segment of DNA (promoter region) that controls expression of GFP.
What is pBAD?
RNA polymerase cannot transcribe GFP.
This chemical makes the cell membrane permeable.
What is CaCl2?
Segment of DNA that encodes for protein repressor of pBAD.
What is araC?
We must add this to release the repressor to transcribe GFP.
What is arabinose?
You will need these three plates to complete the lab.
What are:
LB
LB, Amp.
LB, Amp., ara. (arabinose)