Blood groups
Not enhanced by enzymes
90% of population is negative
What is anti K?
IgM class
What are ABH antibodies?
First phase of testing
What is immediate spin at room temperature?
Used to decrease the zeta potential.
What is low ionic saline solution?
Antibody and red cells interact.
What is sensitization?
Antigens destroyed by enzymes
Null phenotype is resistant to malarial infection
What is Fya and Fyb?
Duffy
H antigen
Inherited independently
precursor to A and B antigens
What is 37C incubation with enhancement media?
Reduces the water in solution to enhance red cell and antibody interaction.
What is PEG?
polyethylene glycol
Test used to assess fetal bleed in Rh negative mothers with Rh positive babies-qualitative
What is the rosette test?
enhanced by enzymes
common cause of delayed transfusion reaction
reactivity in vivo can decrease over time
What is Jka and Jkb?
Kidd
IgG class
What are Rh antibodies?
Performed when DAT is positive.
What is an elution?
Used to enhance or destroy antibody activity.
What are enzymes?
Cells chosen to further testing to rule out/rule in specific antibodies
destroyed by enzymes
shows dosage
Usually not clinically significant
What is MN?
Given to Rh negative mothers who has Rh positive babies
What is Rh immune globulin?
Testing when a patient has a cold autoantibody.
What is a cold autoadsorption?
MN, S, and Duffy
What are antigens destroyed by enzymes?
Stain used to assess fetal bleed in a mother of any blood type-qualitative
What is Kleihaur-Betke stain?
Low incidence antigen
antibodies typically found with full crossmatch only
not usually clinically significant
What is Lutheran a?
L fucose
What is the terminal sugar of the H antigen?
What is DTT treatment?
Increase room temperature incubation of reverse type.
How to enhance weak anti A and B reactions?
Transfusion of a small amount of red cells, stopping the transfusion and observing recipient and vitals, then resuming the transfusion.