General Questions
What is RNA?
RNA, an abbreviation of ribonucleic acid, complex compound of high molecular weight that functions in cellular protein synthesis and replaces DNA (deoxyribonucleic acid) as a carrier of genetic codes in some viruses.
What is organic solvent? Give an example
Organic solvents are carbon-based substances capable of dissolving or dispersing one or more other substances. For example, aliphatic hydrocarbons, aromatic hydrocarbons, amines, esters, ethers, ketones, and nitrated or chlorinated hydrocarbons
What is the essence of the method?
nucleic acids are bound to the membrane by passing the lysate through the membrane using centrifugal force.
Paramagnetics это что (who?)
Это вещества, которые вроде магнитятся, но как бы не совсем. Примерно также как я понимаю статистику
А что, разве у РНК есть secondary structure?
da.
Why should we extract RNA
The extraction of RNA and subsequent analysis with techniques such as RNA-Seq (RNA sequencing) or microarrays will tell one in which functional state the cells
explain the term "elution"
elution is the process of extracting one material from another by washing with a solvent
How is the sample collected at the last stage?
It is collected by centrifugation
What magnetic beads are made from?
Graphene
Зачем добавлять для определения вторичной структуры диметилсульфат?
Он может связываться с неспаренными аденином и цитозином
Because of what RNA is a short-living molecule
Потому что потому
что кончается на у
шучу, из-за рибонуклеаз
Where do we find RNA after centrifuging
The aqueous phase contains RNA.
Name some advantages of this method
Simple, straightforward procedure to perform.
Flexible for use with both centrifugation or vacuum based systems.
Amenable to large-scale and high-throughput processing, including automated methods.
Вопрос- сюрприз
Что такое теория РНК мира и с чем его едят
Хорошая это штука
Хорошо, это шутка
Зачем нам DMS modification in vivo?
чтобы обнаружить аденин и цитозин, который есть только в одинарной цепи
Что, на биотехнолога учишься?
Назови все ncRNA
Abundant and functionally important types of non-coding RNAs include transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as small RNAs such as microRNAs, siRNAs, piRNAs, snoRNAs, snRNAs, exRNAs, scaRNAs and the long ncRNAs such as Xist and HOTAIR.
What is chaotropic agent
It is a substance which disrupts the structure of, and denatures, macromolecules such as proteins and nucleic acids (e.g. DNA and RNA).
Name some disadvantages
Starting with too much sample or incomplete homogenization can clog the membrane and/or result in contamination with proteins or genomic DNA.
Incomplete cellular lysis can lead to low yields.
Automation systems for centrifugation or vacuum can be expensive and complex to set up.
How do we remove pure RNA from the solution with beads?
если коротко, то удерживаем их магнитным полем
различаются ли РНК in vivo и in vitro после ренатурации? Почему?
в клетке вторичную структуру РНК могут изменять белки
Зачем РНК вторичная структура?
Например, для регуляции трансляции
Why could we use guanidinium isothiocyanate?
For cell lysis
What does the buffer from step 2 contain?
RNase inhibitors and a high concentration of chaotropic salts
Какие факторы могут помешать вам при использовании метода?
Potential carry-through of magnetic particles into eluted samples. Slow migration of magnetic particles in viscous solutions. Capture/release of particles can be laborious when performed manually
Вопрос с секретом
Как делают инъекции мышам?
ВнутриМЫШечно