Molecular Biology Methods
This method would allow you purify a protein or protein complex using differential flow rates and a cylindrical column.
Chromatography
This is the prime orientation of the DNA that is used as a template for transcription.
3' to 5'
Subunit of the prokaryotic RNA polymerase that dictates promoter specificity.
Sigma
The cis-acting sequence elements in pre-mRNA that are directly recognized by SNURPs.
5' SS, 3'SS and branch A
This pre-mRNA signal sequence terminates transcription in eukaryotes and is bound by CPSF.
AAUAAA
This method would allow you to quantify the levels of a specific mRNA in a cell/tissue in "real-time".
RT-qPCR
The general term for the cis-acting signal sequence that dictates the start of transcription (unstream or flanking the +1 site).
Promoter
Highly-conserved protein complexes that determine DNA accessiblity; can be chemically modified in their N-termini.
Histones
In general, these are regions of pre-mRNA that are removed via the splicing process.
Introns
What are 2 reasons that eukaryotes cap their mRNAs?
Any 2 are correct: Nuclear export, stability, translation, gene expression regulation
This method would allow you to quantify the levels of all mRNA in an organisms cell/tissue.
RNA-seq
The site of DNA that can be bound by a repressor protein in prokaryotes.
Operator
Once thought to function more as a gene-specific transcription factor, this heterogeneous multi-subunit protein complex is now an important part of the basal eukaryotic RNA polymerase II machinery.
Mediator
The names of the 5 different SNURPs.
U1, U2, U4/5/6
Capping requires these 3 enzyme activities.
RNA triphosphatase, guanyltransferase, and methyltransferase
A radioisotope you'd use to label and track newly synthesized proteins.
35-S
These regions of eukaryotic DNA can be far away from the core promoter; they are bound by gene-specific transcription factors.
Enhancers
This protein can facilitate termination of transcription in prokaryotes, but it doesn't appear to be critical.
Rho
Which are longer: Introns or Exons?
Introns
The 5' cap structure is missing this "Greek letter" type of phosphate.
Gamma
This method allows you to quantify the levels of a specific protein in a sample. It relies on several antibodies, a 96-well microtiter plate, and a plate reader.
ELISA
A regions of eukaryotic DNA that is bound by the TBP protein; once thought to be a conserved promoter element and now known to be missing for some genes.
The C-terminal domain of this eukaryotic protein controls the rate of transcription, and also is a binding platform for splicing, capping, and poly-A factors.
Rpb1
These trans-acting factors regulate SNURP activity to dictate alternative splicing of pre-mRNAs.
SR proteins and hnRNPs
True or False: Alternative polyadenylation can lead to transcripts with longer 3'UTRs.
True