Aseptic Techniques
The purpose of streaking for isolation
What is producing isolated colonies of an organism on an agar plate?
Name one gram positive and one gram negative bacteria.
E coli- gram -
Bacillus- gram +
*know ex of eukaryotic, non-pathogenetic, anearobic/aerobic, enteric, pond water*
What is an experimental and control variable? Provide an example.
What are variables staying the same through each trial vs. variables being tested?
Gram stains can differentiate bacteria based on their....
What are cell walls (peptidoglycan)?
What are chemical agents that kill or control microbes on inanimate surfaces?
Ex: alcohol
Name a technique used to view a microorganism
What is wet mount technique?
What is transient flora?
What is foreign bacteria?
What is the format of a scientific hypothesis?
"If x, then x"
The hypothesis should relate back to your original question and must be testable
MAC agar selects for _____ ______ bacteria and differentiates based on______ ________.
What are gram negative bacteria and lactose fermentation?
How is antibiotic resistance created?
Mis and overuse: Bacteria mutate and develop resistance. They remain and multiply, so the more antibiotics we use, the less effective they are.
Name two streaking techniques we did in lab
What are isolation and lawn streak techniques?
Name an anerobic, gram positive bacteria
What is Clostridium sporogenes?
What is 780,000,000 in scientific notation?
7.8x10^8
Name a Staphylococcus that has an alpha hemolysis on BAP
What are S. pneumoniae and/or S. viridans?
*know what hemolysis looks like*
Which antibiotics are more resistant and most susceptible?
***look at photo!***
Draw a sterile area around the bunson burner
(sterile ~50cm area, also note the hot/cold air)
What microbe is associated with dental caries?
What is Streptococcus mutans?
What is generation time? If MRSA has a faster generation time than E coli, would MRSA have more or less colonies?
What is the time it takes bacteria to double in population?
More!
S. pyogenes (GAS) hemolysis on BAP
What is gamma?
Define MIC
Minimum inhibitory concentration is lowest concentration of antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation
What is it called when a microbe accidentally gets into your culture
Name an enteric microbe and where in the body you may find it.
What is Enterococcus faecalis that resides in the gut?
You plate 0.1 mL of a 10^-6 dilution, and the plate has 67 colonies. Calculate original CFU.
67 CFU/0.1mL=670CFU/mL
(670CFU/mL)/10^-6= 6.7*10^8
TRIPLE SUGAR IRON AGAR (TSIA) tests for 3 reactions. Name them.
What is hydrogen sulfide and the fermentation of glucose, lactose and/or saccharose?
red/red- negative
red/yellow- positive for glucose
yellow/yellow- positive for glucose and lactose/sucrose
Blackening- positive for hydrogen sulfide
Name a disinfectant, an antibiotic, and a form of moist heat.
lysol, penicillin, and steam