Amino Acids
The side chains of these two amino acids are positively charged at physiological pH.
What are lysine and arginine?
This type of enzyme catalysis involves the transfer of a proton to and from a reactive group of the substrate or enzyme.
What is acid-base catalysis?
This type of membrane lipid has a steroid ring structure and is an important regulator of membrane fluidity in animals.
What is cholesterol?
This hydroxyl group containing amino acid also contains a phenyl ring and is thus classified as an aromatic residue.
What is tyrosine?
This reaction intermediate has the highest free energy and is thus the least stable intermediate formed during an enzyme-catalyzed reaction pathway.
What is the transition state?
This technique can be used to study the diffusion of a lipid or protein within a lipid bilayer or membrane and involves the use of a fluorescent tag.
What is FRAP (fluorescence recovery after photobleaching)?
This amino acid often acts as an acid catalyst in enzyme-catalyzed reactions, although it is classified as a basic amino acid.
What is lysine?
This Michaelis-Menten model assumption holds that the concentration of the enzyme-substrate complex remains constant during the course of a reaction.
What is the steady-state assumption?
Bacteriorhodopsin would be classified as this type of membrane protein because it interacts extensively with the lipid bilayer and requires the use of harsh chemicals to be released from membranes.
What is an integral membrane protein?
These two amino acids are rarely found in elements of secondary structure due to the unique conformational properties of their peptide backbone bonds.
What are glycine and proline?
This Michaelis-Menten kinetic parameter is a measure of the number of reaction cycles catalyzed by an enzyme per unit time when the enzyme is saturated with substrate.
What is kcat (the turnover number)?
Free fatty acids and single-tail detergents tend to form these types of structures in aqueous solutions due to the hydrophobic effect.
What are micelles?
These two sulfur containing amino acid residues have important roles in the initiation of translation and a variety of enzyme-catalyzed reactions.
What are methionine and cysteine?
The catalytic mechanism of carbonic anhydrase involves the use of this metal ion to activate a water molecule for nucleophilic attack.
What is zinc?
This type of lipid diffusion event is rare and requires the polar portion of a lipid to travel through an otherwise nonpolar environment.
What is flip-flop (transverse) diffusion?