Gram-stain
Crystal Violet
Endospores stain are what colors?
Green and red
What type of googles do we use in lab?
Z87
Best stain to observe morphology
Negative
What is the counter stain for gram-stain?
Safranin
What is the counter stain?
What is the primary stain?
Safranin - Malachite green
What does FTM media test?
Aerotolerance! What is aerotolerance?(EC)
The rack
Primary stain in capsule stain?
Carbolfuchsin - stains pink
What are two possible decolorizers? What do they do?
Ethanol or acetone - alcohols - remove LPS layer
What is the function of an endospore? What color does the endospore stain?
Survival - protect DNA
What do we call organisms that love C02?
Capnophiles
What is the function of immersion oil?
Account for light refraction.
Can someone explain?
Counter stain for capsule stain?
what does it stain and what color?
Methylene blue - blue - stains the non acid fast
What is Iodine used for? What is it's specific MOA?
Does this technique use a rinsing agent or decolorizer?
Rinsing agent - water
Difference between anaerobic and aerobic?
Anaerobic: No oxygen
Aerobic: Need oxygen
Where is the gas shut off button?
Back wall by the door
What is the pH of the cell wall?
Acidic by nature
Why does the Gram-Stain work?
Because of the different compositions of the cell wall.
Explain?
What type of cells produce endospores?
Gram-Postive Rods - GPR
What is ambient growth?
Growth in a normal environment
On any reagents we use in class there are 4 colors each with a meaning. What are the colors and their meanings?
Blue- health
red - flammability
Yellow- reactivity
White - personal
What is in acid fast stains the make them stain pink?
Mycolic acid