Structure
What class of biomolecules is an enzyme?
What are proteins?
According to Beers Law, the absorbance of a solute is directly proportional to what in solution?
What is the concentration of solute?
Name two things that affect function.
What are temperature, pH, substrate concentration, enzyme concentration, presence of inhibitors or activators.
In what types of cells are these enzymes found in?
What are all living cells?
Over 100 years ago, biologists found that animals’ immune systems respond to invasion by these “foreign entities” on infectious agents?
What are antigens?
In the immune system, these are used to recognize antigens like disease agents.
What are primary antibodies?
What is the function of Taq DNA Polymerase in a PCR reaction?
What is synthesizing new DNA strands by adding bases to primer sequence?
What is the function of superoxide dismutase?
What is breaks down harmful oxygen radicals in cells that cause damage to tissues?
What is the structure in an enzymatic reaction with the highest amount of energy?
What is the transition state?
What does a solvent travel through in column chromatography to separate associated molecules?
What is the stationary phase?
What is the function of the lacZ gene in bacteria?
What is to allow the bacteria to use lactose as a food source?
What type of enzyme is this?
What are antioxidant enzymes?
In our experiment, what specific enzyme did we use to study the nature of enzymes and how do we know the reaction proceeded?
What is cellobiose, and it changed from clear to yellow?
To what does Coomassie dye bind to in a protein to change its color to an intense blue?
What are hydrophobic amino acids?
What was the first enzyme to be crystallized and solved by x-ray crystallography, which is now used as a model protein for introductory crystallization experiments?
What is lysozyme?
What does it catalyze the dismutation of?
What is a superoxide anion to a hydrogen peroxide (H2O2) and triplet oxygen (O2-)?