Canada and Hamiltonians
The year Canada wants to reach Net Zero?
2050
This enzyme is involved in the rate limiting step in the MEP?
SQE
These 2 hamilton industries can be targeted for our fuel source in the biosystem
Steel plant/burners
Breweries
The precursors for the ergosterol biosynthesis pathway are made in this cellular location
Chloroplast
The two causes of progesterone shortages in canada
aging Canadian population
Climate change impacts the crop and supply chain
What does GMP stand for in regulation processes?
good manufacturing process
It is this percentage of the sterol profile that is made of ergosterol in chlamydomonas reinhardtii?
63%
How did Chloronova influence this years project?
Carbon capture model using hamilton industry
This happens to engineered strains after a few generations
returns to parent
Even though yeast is much faster at making ergosterol, what three reasons do we believe our system is viable against it
Low cost
Carbon capture
Locality to needed populations
This percentage of women over the age of 55 experience menopausal symptoms (same number as those who are then prescribed HRT to alleviate these symptoms)
75-85%
This is the method of transformation for our algae strain?
Glass Bead
This type of difference is expected in the ergosterol profile when endogenous enzymes are expressed
Null - none
This is where we will expect our gene fragment to integrate
nuclear genome
Algae biologist believe industrialists use algae as a greenwashing technique because of this change in scale up
Acetate as the carbon source
The earliest we can see any changes, big or small in the steel plants is 2060 because of this economic reason
Burner warranty being 25-50 years old
These are the three key features of our strain ordered from crc
Hint - cw15 not1, nit2, Arg7-8
Cell wall deficient
Arginine auxotroph
Selective growth on nitrogen containing media
What percent of carbon does our biosystem need to fuel the algae?
12% industry
5% in lab
This is the main difference between a kinetic model vs a flux balance analysis
The unit over time
Enzyme concentrations vs reaction activity
This economic factor stopped the successful uptake of algae biofuel in 2010’s
Biofuel cost against natural gas cost
What percent of progesterone is used in medicines dispensed?
30 percent in capsules,
8 percent in creams
This flaw in cyanobacteria stopped us from choosing it as a chassis to make sterols
Sterol profile
What percent of biomass is expected to be ergosterol?
0.1%
This was a concern for design given that two transcription units that use the same promoter were right next to eachother in our circuit
Transcriptional interference
Competition for machinery
What is the current for carbon credits today in the form sequestered carbon?
100$/tonne