Specimen collection
Most common site for collection of blood sample
Antecubital fossa
Preferred sites for ARTERIAL PUNCTURE
Radial artery at wrist
The first urination is discarded but all urine after this time is collected.
24 hour urine specimen collection
What color Stopper contains the additive 'Heparin"
Green
Site for Lumbar puncture
Between L3-L4
Which part of blood contains fibrinogen
Plasma
What type of syringe is preferred for ABG specimen collection?
Pre-heparinized plastic or glass syringe
Preservatives for 24hr urine for analysing inorganic phosphate, Calcium
HCl
Which additive is a glycolytic inhibitor
Sodium fluoride
What preservative is used for Fecal sample
None, keep the container refrigerated throughout collection
What is the recommended alcohol to clean puncture sites?
70% isopropyl alcohol
Why is it important to expel air bubbles from the ABG syringe immediately after collection?
Air bubbles can alter pO₂ and pCO₂ values
Storage time of sample post analysis
36-48 hrs
This color tube is the last tube to draw in the order of draw for venipunctures.
Grey
Samples to be transported in Ice
Ammonia , ABG
Tourniquet should be placed _______ inches above the antecubital space
3
What test must be performed before puncturing the radial artery to ensure collateral circulation?
Allen’s test
How are urine specimens discarded
By addition of hypochlorite solution and flushing
Red capped vaccutainer contains which anticoagulant
None
A fluid sample received in the lab is labeled “body fluid,” but no site is mentioned. What are the steps the technician should take upon receiving an unidentified fluid sample? How does labeling affect interpretation?
Confirm with clinician, relabel after clarification. Labeling ensures correct interpretation for fluid type
What can increase the blood flow when doing the capillary puncture?
Warming the site for capillary puncture
An ABG sample collected for ICU patient was transported without ice and analyzed after 20 minutes. How does delayed analysis affect blood gas results, and what are the correct storage and transport guidelines?
Delay raises CO2, lowers pO2. Transport on ice, analyze within 10–15 min.
Urine specimen completely free of extraneous contamination is
Suprapubic Aspiration
A potassium EDTA tube is mistakenly used for serum potassium estimation. What analytical error will occur.
EDTA causes false high K+, low Ca and Mg.
Amniocentesis is done to diagnose
Prenatal diagnosis of congenital disorders