Microscopes
How do you calculate total magnification?
Magnification of objective lens x magnification of eye piece lens (10x)
pregnancy screen, drug screen, strep test, occult blood
What does CDC stand for? What does it do for labs?
Centers for Disease Control-helps oversee public health and infectious disease
What does POC stand for?
Point of Care
True or False, all clinical labs need to participate in proficiency testing.
True if they are conducting tests classified as moderately to highly complext tests
Which objective needs to have oil applied to the slide prior to using?
100x objective
What is a waived test?
A lab test that anyone can do provided they were trained, has fairly simple instructions, low risk to patient care
What does FDA stand for and which area of lab does it oversee?
Food and Drug Administration-looks at blood banking
Which POC test might use a small handheld device?
Glucometer/ glucose meter
INR
Which 2 lab positions are required to do PT?
MLT's and MT's
What do you do with the stage prior to looking at a wet mount?
Lower the condenser and/or stage to be able to focus
Why do labs use external QC for waived tests?
To ensure the kits are working as expected prior to patient testing.
What does CMS stand for?
Center for Medicare and Medicaid Services. Oversees all human patient healthcare and testing in the US.
What is a potential disadvantage of a POC test?
Cost-some can be costly if include instrumentation, purchasing of separate controls, reagents, service agreements
What are 2 things to check for upon receiving proficiency testing shipment?
Correct Temperature and volume (correct number of vials and sufficient volume)
When do you set up the scope as "high and dry?"
When looking at a wet mount
Why is a rapid strep test performed? Please state the bacteria tested for and a possible complication if not treated.
Testing for Group A strep. If not treated, can result in sequelae such as infection of cardiac valves, rheumatoid arthritis, scarlet fever
Why is CLIA-88 important to the labs?
CLIA-88 creates the standards and guidelines for clinical laboratory testing.
What does occult blood test for?
Presence of blood in stool.
What is the name of the form testing personnel sign when they complete proficiency testing?
Attestation form
What happens to the image in the field of view as magnification increases?
The detail increases, but you look at a smaller section of the original field of view (you are focusing in on a piece of the original image)
Please compare moderately complex lab testing to waived testing. State 3 key differences.
Waived testing-anyone can do provided they are trained, while only MLT's and MT's can do moderately complex testing
Moderately complex testing is more difficult, may include automation. Waived tests are simple, can include use of instrument.
Moderately complex tests typically require interpretation based on observations and/or calculations. Waived testing does not require difficult interpretation (clearly guidelines show what different results are)
Which agency dictates a lab has to undergo proficiency testing?
CLIA-88 (on behalf of CMS)
POC tests can only be used at patient bedside, True or False
False. Many times a POC test is used as a screening test only in the lab, although they have capacity to be used at patient bedside.
How many times per year does a lab do proficiency testing.
Three shipments per year= 3 times per year. Need to be rotated among staff