Anesthesia
A patient shows pain in response to light touch of an area that would not normally be painful. Which term best describes this finding?
A. Hyperalgesia
B. Allodynia
C. Nociception
D. Analgesia
A patient shows pain in response to light touch of an area that would not normally be painful. Which term best describes this finding?
A. Hyperalgesia
B. Allodynia
C. Nociception
D. Analgesia
Which ultraviolet range is most associated with germicidal activity?
A. UV-A
B. UV-B
C. UV-C
D. Visible light
Which ultraviolet range is most associated with germicidal activity?
A. UV-A
B. UV-B
C. UV-C
D. Visible light
Which of the following is the only finding that, by itself, confirms death?
A. Absence of corneal reflex
B. Graying of mucous membranes
C. Rigor mortis
D. Absence of palpable pulse
Which of the following is the only finding that, by itself, confirms death?
A. Absence of corneal reflex
B. Graying of mucous membranes
C. Rigor mortis
D. Absence of palpable pulse
Which organization’s mission is to improve the welfare of animals in science and education through accreditation of institutions meeting high standards of animal care and use?
A. ACLAM
B. AAALAC International
C. AALAS
D. OLAW
Which organization’s mission is to improve the welfare of animals in science and education through accreditation of institutions meeting high standards of animal care and use?
A. ACLAM
B. AAALAC International
C. AALAS
D. OLAW
The Recombinant DNA Advisory Committee (RAC) was established primarily to:
A. Accredit animal care and use programs internationally
B. Enforce Good Laboratory Practice regulations
C. Alleviate public concern and review human gene transfer research
D. Serve as the NIH institutional compliance office for animal welfare
The Recombinant DNA Advisory Committee (RAC) was established primarily to:
A. Accredit animal care and use programs internationally
B. Enforce Good Laboratory Practice regulations
C. Alleviate public concern and review human gene transfer research
D. Serve as the NIH institutional compliance office for animal welfare
Which portion of the pain pathway is most directly associated with conversion of a noxious stimulus into neural activity at the peripheral nerve ending?
A. Modulation
B. Perception
C. Transmission
D. Transduction
Which portion of the pain pathway is most directly associated with conversion of a noxious stimulus into neural activity at the peripheral nerve ending?
A. Modulation
B. Perception
C. Transmission
D. Transduction
Which containment layer includes a biosafety cabinet?
A. Tertiary containment
B. Primary containment
C. Secondary containment
D. Administrative containment
Which containment layer includes a biosafety cabinet?
A. Tertiary containment
B. Primary containment
C. Secondary containment
D. Administrative containment
Which statement about nitrogen or argon euthanasia is most accurate?
A. It is acceptable for all mammals because it is non-aversive
B. It is acceptable with conditions for chickens, turkeys, and pigs when oxygen is kept below 2% for more than 7 minutes
C. It is preferred in neonatal rodents because recovery cannot occur
D. It is recommended for routine euthanasia of conscious laboratory mice
Which statement about nitrogen or argon euthanasia is most accurate?
A. It is acceptable for all mammals because it is non-aversive
B. It is acceptable with conditions for chickens, turkeys, and pigs when oxygen is kept below 2% for more than 7 minutes
C. It is preferred in neonatal rodents because recovery cannot occur
D. It is recommended for routine euthanasia of conscious laboratory mice
Which federal regulation contains the Good Laboratory Practice standards overseen by FDA?
A. 9 CFR
B. 21 CFR Part 58
C. 29 CFR 1910
D. 42 CFR Part 73
Which federal regulation contains the Good Laboratory Practice standards overseen by FDA?
A. 9 CFR
B. 21 CFR Part 58
C. 29 CFR 1910
D. 42 CFR Part 73
Which of the following best meets the NIH Guidelines threshold for requiring a Biosafety Officer?
A. Any BL2 recombinant DNA experiment
B. Any experiment involving transgenic rodents
C. Research conducted at BL3/4 or large-scale work exceeding 10 liters
D. Any experiment reviewed by an IBC
Which of the following best meets the NIH Guidelines threshold for requiring a Biosafety Officer?
A. Any BL2 recombinant DNA experiment
B. Any experiment involving transgenic rodents
C. Research conducted at BL3/4 or large-scale work exceeding 10 liters
D. Any experiment reviewed by an IBC
Which of the following is correctly matched?
A. NMDA receptor — fast, transient excitatory signaling
B. AMPA receptor — slow, sustained excitatory signaling
C. Glycine — primary excitatory neurotransmitter
D. Glutamate — primary excitatory neurotransmitter
Which of the following is correctly matched?
A. NMDA receptor — fast, transient excitatory signaling
B. AMPA receptor — slow, sustained excitatory signaling
C. Glycine — primary excitatory neurotransmitter
D. Glutamate — primary excitatory neurotransmitter
Which statement best distinguishes biosafety levels from risk groups?
A. Biosafety levels describe the agent only, while risk groups describe the facility only
B. Risk groups describe the agent, while biosafety levels reflect the containment practices and facility features used for the work
C. Risk groups are based only on airflow, while biosafety levels are based only on PPE
D. They are interchangeable terms
Which statement best distinguishes biosafety levels from risk groups?
A. Biosafety levels describe the agent only, while risk groups describe the facility only
B. Risk groups describe the agent, while biosafety levels reflect the containment practices and facility features used for the work
C. Risk groups are based only on airflow, while biosafety levels are based only on PPE
D. They are interchangeable terms
Which of the following pairings is correctly matched for euthanasia of laboratory mice?
A. Ethanol, 0.5 mL of 70–100% IP — acceptable with conditions in adult mice not less than 35 days old
B. Nitrogen/argon — acceptable with conditions for adult mice because it is non-aversive
C. Potassium chloride — acceptable as a sole agent if given intraperitoneally
D. Urethane — acceptable with conditions for nonsurvival studies only
Which of the following pairings is correctly matched for euthanasia of laboratory mice?
A. Ethanol, 0.5 mL of 70–100% IP — acceptable with conditions in adult mice not less than 35 days old
B. Nitrogen/argon — acceptable with conditions for adult mice because it is non-aversive
C. Potassium chloride — acceptable as a sole agent if given intraperitoneally
D. Urethane — acceptable with conditions for nonsurvival studies only
"Which statement about record retention is correct?
A. OSHA Form 300 records must be retained for 2 years
B. DEA inventories and required records must be kept at least 2 years
C. GLP records are always kept exactly 2 years regardless of study outcome or FDA submission
D. APHIS annual reports and DEA Form 222 records are both retained for three years"
"Which statement about record retention is correct?
A. OSHA Form 300 records must be retained for 2 years
B. DEA inventories and required records must be kept at least 2 years
C. GLP records are always kept exactly 2 years regardless of study outcome or FDA submission
D. APHIS annual reports and DEA Form 222 records are both retained for three years"
DEA inventories and related records are kept at least 2 years, while OSHA Form 300/300A records are kept 5 years following the applicable year.
Which statement correctly distinguishes NIH Guidelines Section III-D from Section III-E?
A. Section III-D requires NIH Director approval, whereas Section III-E is exempt from IBC registration
B. Section III-D requires IBC approval before initiation, whereas Section III-E requires IBC notice simultaneous with initiation
C. Section III-D applies only to human gene transfer, whereas Section III-E applies only to whole plants
D. Section III-D is limited to BL1 work, whereas Section III-E applies only above BL2
Which statement correctly distinguishes NIH Guidelines Section III-D from Section III-E?
A. Section III-D requires NIH Director approval, whereas Section III-E is exempt from IBC registration
B. Section III-D requires IBC approval before initiation, whereas Section III-E requires IBC notice simultaneous with initiation
C. Section III-D applies only to human gene transfer, whereas Section III-E applies only to whole plants
D. Section III-D is limited to BL1 work, whereas Section III-E applies only above BL2
Which volatile anesthetic is associated with the greatest myocardial sensitization to catecholamines?
A. Nitrous oxide
B. Isoflurane
C. Sevoflurane
D. Halothane
Which volatile anesthetic is associated with the greatest myocardial sensitization to catecholamines?
A. Nitrous oxide
B. Isoflurane
C. Sevoflurane
D. Halothane
Which disinfectant is active in vapor phase, and non-toxic, but dependent on environmental moisture for efficacy?
A. Sodium hypochlorite
B. Peracetic acid
C. Hydrogen peroxide
D. Chlorine dioxide
Which disinfectant is active in vapor phase, and non-toxic, but dependent on environmental moisture for efficacy?
A. Sodium hypochlorite
B. Peracetic acid
C. Hydrogen peroxide
D. Chlorine dioxide
Which species has IV barbiturates as the only acceptable method?
A. Rabbits, swine, ruminants, and birds
B. Dogs, cats, swine, and fish
C. Rabbits, poultry embryos, rodents, and amphibians
D. Nonhuman primates, fish, birds, and pigs less than 3 days old
Which species has IV barbiturates as the only acceptable method?
A. Rabbits, swine, ruminants, and birds
B. Dogs, cats, swine, and fish
C. Rabbits, poultry embryos, rodents, and amphibians
D. Nonhuman primates, fish, birds, and pigs less than 3 days old
A residency program has 6 ACLAM residents enrolled simultaneously. Based on the training program recognition criteria, what is the minimum number of affiliated ACLAM Diplomates required?
A. 1
B. 2
C. 3
D. 4
A residency program has 6 ACLAM residents enrolled simultaneously. Based on the training program recognition criteria, what is the minimum number of affiliated ACLAM Diplomates required?
A. 1
B. 2
C. 3
D. 4
Programs must have at least one affiliated Diplomate per three residents.
Which scenario is most likely to be exempt under Section III-F of the NIH Guidelines?
A. A BL2 experiment involving a transgenic rodent expressing more than half of an exogenous viral genome
B. A human gene transfer protocol using recombinant viral vectors
C. A study transferring antibiotic resistance between strains of E. coli by natural bacterial conjugation
D. A large-scale culture experiment involving more than 10 liters of recombinant material
Which scenario is most likely to be exempt under Section III-F of the NIH Guidelines?
A. A BL2 experiment involving a transgenic rodent expressing more than half of an exogenous viral genome
B. A human gene transfer protocol using recombinant viral vectors
C. A study transferring antibiotic resistance between strains of E. coli by natural bacterial conjugation
D. A large-scale culture experiment involving more than 10 liters of recombinant material
Nitrous oxide’s “second gas effect” is primarily related to which property?
A. High blood solubility
B. High partial pressure delivery with rapid uptake from the alveoli
C. Extensive hepatic metabolism
D. High oil/gas partition coefficient
Nitrous oxide’s “second gas effect” is primarily related to which property?
A. High blood solubility
B. High partial pressure delivery with rapid uptake from the alveoli
C. Extensive hepatic metabolism
D. High oil/gas partition coefficient
Which best describes a Class II Type B2 biosafety cabinet?
A. Provides personnel protection only, with inward airflow and no product protection
B. Recirculates approximately 70% of air within the cabinet and exhausts about 30%
C. Exhausts approximately 70% of air and recirculates about 30% within the cabinet before discharge
D. Is hard-ducted and 100% exhausted, with no recirculation within the cabinet
Which best describes a Class II Type B2 biosafety cabinet?
A. Provides personnel protection only, with inward airflow and no product protection
B. Recirculates approximately 70% of air within the cabinet and exhausts about 30%
C. Exhausts approximately 70% of air and recirculates about 30% within the cabinet before discharge
D. Is hard-ducted and 100% exhausted, with no recirculation within the cabinet
Which euthanasia agent is correctly matched to its drugs?
A. Tributame — mebozonium iodide, tetracaine hydrochloride, embutramide
B. T-61 — embutramide, chloroquine phosphate, denatonium benzoate
C. Tributame — embutramide, lidocaine, chloroquine phosphate
D. T-61 — embutramide, lidocaine, denatonium benzoate
Which euthanasia agent is correctly matched to its drugs?
A. Tributame — mebozonium iodide, tetracaine hydrochloride, embutramide
B. T-61 — embutramide, chloroquine phosphate, denatonium benzoate
C. Tributame — embutramide, lidocaine, chloroquine phosphate
D. T-61 — embutramide, lidocaine, denatonium benzoate
Tributame is embutramide, lidocaine, and chloroquine phosphate, whereas T-61 contains embutramide, tetracaine hydrochloride, and mebozonium iodide
Which OSHA document serves as the running annual log of work-related injuries and illnesses?
A. Form 300A
B. Form 301
C. Form 300
D. First Report of Injury only
Which OSHA document serves as the running annual log of work-related injuries and illnesses?
A. Form 300A
B. Form 301
C. Form 300
D. First Report of Injury only
Within the NIH framework presented, which office is responsible for reviewing and coordinating activities related to the NIH Guidelines and for oversight of dual-use research of concern?
A. OLAW
B. OSP
C. OBA
D. RAC
Within the NIH framework presented, which office is responsible for reviewing and coordinating activities related to the NIH Guidelines and for oversight of dual-use research of concern?
A. OLAW
B. OSP - office of science policy
C. OBA
D. RAC