2200 Exam 3

Trouble-shooting

100

Gram positive cocci

Negative rapid strep

Beta colonies on blood agar

What is Streptococcus pyogenes or positive.

200

Cause for positive test pad and negative control pad of lateral flow.

What is expired or old secondary antibody.

300

Anti A: Positive

Anti B: Positive

Anti D: Positive

A cell: Negative

B cell: Negative

Rh Control: Positive 

Blood type?

What is discrepant or spontaneous agglutination?

400

1. Horse RBCs

2. Patient serum

3. Guinea pig antigen

4. Mix (1) + (2)

5. Mix (1+2) + (3) into quarter sized area.

6. Clear blue background with many clumps.

Interpret.

What is false positive?

500

Anti-A:  Negative

Anti-B: Negative

Anti-D: Positive

A cell: Positive

B cell: Negative

Blood type.  If discrepant provide cause.

Discrepant.  Type B but forward not working.  Due to no anti-sera added.

Pieces

100

Forward D Typing Components

What are Anti-D and patient RBCs?

200

EBV agglutination testing components.

What are

Horse RBCs,

Guinea Pig Ag,

Patient serum or plasma

300

Rapid strep test components.

What are

Throat swab;

primary Ab-conjugate (sample pad)

Primary Ab (test pad)

Secondary Ab (control pad)

400

Reactive lymphocyte components

What are

1. Large lymphocyte

2. Scalloped edges

4. Blue edges

5. Pulled nucleus or homogenous smooth chromatin

500

1. B cell

2. Plasma cell

3.Antibody

4. Antigen removal.  How?

What is

1. Neutralize

2. Opsonize

3. Lysis

Maybe I will like micro someday?

100

Organelle present in both eukaryotes and prokaryotes.

What are ribosomes?

200

Lyme disease causing bacterium

What is borrelia?

300

Tularemia and rabbits

What is Francisella?

400

Fungi stain detection method.

What is calcofluor white or lactophenol cotton blue?

500

Microscopic detection of parasites.

What is trichome stain or iodine wet prep?

Potpourri

100

Source of blood agar RBCs.

What are sheep?

200

Micro dilution Testing:

Cloudy: 0.1, 0.2, 0.4, 0.8

Clear: 1.6, 3.2 

MIC?

What is 1.6

300

Infection caused by being in a hospital setting

What is nosocomial infection?

400

Rh Control composition 

What is antisera without antibody

500

3 classes of parasites

What are

Arthropod, helminth, protozoa

Potpourri 2

200

32 mm measurment

Reference:

31

Interpet.

Susceptible

300

A tech applied 95% ethanol for 1 minute during gram staining.  What is result of E.coli?

What is no effect?

400

Sugars....help differentiate ABO antigens.

What are

H Ag = Fucose

A Ag = GalNAC

B Ag = Galactose

500

Anti-A: Negative

Anti-B: Negative

Anti-D: Negative

Ac: Positive

Bc: Negative

Blood type?  If discrepant, provide cause.

What is discrepant due to weak antibody?