Category 1
True or False: Glassware, solutions, or other types of apparatus should never be lifted above eye level.
True
The difference between the cell viability when it comes to primary vs. cell lines.
Primary cells -- finite life span in vitro
Cell line -- indefinite (immortalization/transformed)
The reason why the media has a pink color.
What is due to phenol red?
The two types of light microscopes.
What is simple (one convex lens) and compound (two optical parts)?
Another name for stereo microscopes.
What are dissecting microscopes?
CRISPR is found in _______.
What is bacteria?
FISH stands for
What is fluorescent in situ hybridization?
CISH stands for
What is chromogenic in situ hybridization?
This is used to convert RNA back to cDNA.
What is reverse transcriptase?
Microscope that has low magnification power and is the only microscope that can be used on living samples.
What are stereomicroscopes?
The chemical used to make the gradient for cell dissociation.
What is Percoll gradient?
Organoid cultures are induced by these.
Meaning when the color of media switches from red --> orange.
What is need to change the old media?
Organoid cultures are derived from these.
What are embryonic stem cells?
Being able to study cell-cell interaction is an advantage of this.
What is co-cultures?
Allows for reversible silencing of the gene expression - helpful for genes where a total loss of function is lethal or for studying dosage effects of genes.
What is CRISPRi (interference)?
Increases gene expression and uses dCas9
What is CRISPRa (activation)?
The two major components for in situ hybridization.
What is target DNA and probe?
The type of RNA that is naturally occurring in plants and animals' genomes.
What is miRNA?
The type of RNA that is synthetically produced.
What is siRNA?
Uses for flow cytometry.
The challenges involved in cell dissociation -- name at least three.
What is maintaining the viability of the cells, inconsistencies in batch of chemicals used for cell dissociation, various protocols available for cell dissociation, inconsistencies in processing the tissue samples, and avoiding cross contamination of other cell types?
List the four types of chemical contaminants.
Name three of the components of the basal media.
What are inorganic salts, Phenol red, buffers (bicarbonate and HEPES), glucose, ketoacid (oxalaceate and pyruvate), and carbohydrates?
Name at least three advantages of cell dissociation.
Cell type specific studies can be carried, cell-cell interaction can be studies using co-cultures, organoid cultures can be established, tumor-sphere cultures can be established, and cell lines can be established.