Types of NP Formulations
What is a nanoparticle?
Particles used to deliver drugs/proteins to a target site
What is this image showing?
The molecules that go into the microfluidic device so that functional assays can be performed.
List two types of tests conducted in this study.
Permeability and nanoparticle tests
What therapeutic agent is used in this study?
Nanoparticles
How could one of the concepts from the article apply to your tissue engineering solution?
Many answers
What are the three NP formulations investigated in this study?
AP2 NPs, pPLD NPs, and Bare NPs
What can be said about LRP1?
LRP1 signaling is lowest when there is no GBM spheroid, then an increase in LRP1 signaling is seen far from the GBM spheroid, and the most LRP1 signaling is seen near the GBM
What type of test was conducted to determine paracellular permeability? What molecule is fluorescence applied to?
Confocal microscopy and dextran
What is the gold standard treatment for glioblastoma (before microfluidic device)?
Chemotherapy, radiation, and surgery
What other organs in the the body have better permeability than the brain?
Ovaries and lungs (threefold)
What method of transportation allows the AP2 NPS to cross the BBB? What test supports this hypothesis the best?
LRP1-mediated transcytosis and test to show affect was temperature change in 37 C and 21 C
What does the figure reveal about the effect of GPM proximity and LRP1 on nanoparticle transport across the BBB?
LRP1-mediated transcytosis is potentially responsible for enhanced nanoparticle transport in proximity to GBM.
How were the CDDP formulations in the GBM cells tracked? What type of CDDP NP had the strongest signal based off the setup of the test?
Sytox nucleic acid stain to label dead cells in the three ROIs (far, near, and inside GBM spheroids) and AP2 CDDP NPs had strongest signal inside the tumors
What two particles make-up the GBM spheroids? And in what ratio?
GBM22 cells & pericytes, in a 4:5 ratio
How do pericytes and astrocytes contribute to the BBB for studying glioblastoma?
Pericytes help with blood vessel formation and astrocytes make up the majority of the cells in the CNS and regulate blood flow.
How does it evaluate the differential permeability and therapeutic efficacy of bare nanoparticles, pPLD nanoparticles, and AP2-functionalized nanoparticles in the in vitro BBB-GBM model and in vivo mouse models?
The study evaluates the permeability and therapeutic efficacy of different nanoparticle formulations by measuring their ability to cross in vitro and in vivo BBB and their subsequent impact on tumor growth and cell viability in glioblastoma models.
What can be seen in this figure?
Dextran permeability in the microvascular networks (MVN) and BBB of mice depending on the NP formulation.
How do permeability rates of 40kDa and functionalized particles across the BBB in the microfluidic model compare with those in live mouse models?
Two-photon microscopy, dextran permeability assays, and nanoparticle permeability assay.
What treatment was being tested of the LBL-NPs for GBM to observe the influence of drug delivery? Why was this treatment plan selected?
CDDP sent to GBM tumors and CDDP was used due to its nonspecific mechanism of action and its poor BBB penetration
What is a limitation of this study?
The in vitro BBB-GBM model does not have continuous flow.