Gene Expression
Technology
Gene Function
100

Complementary mRNA

3‘ A-C-T-T-G-T-A-A-C-A 5’

What is 5‘ U-G-A-A-C-A-U-U-G-U 3’

100

What enzyme makes cDNA from mRNA

What is reverse transcriptase
100

What is the primary function of CRISPR-Cas9

What is cutting DNA for gene editing

200
What molecule indicates that a gene is actively being expressed in a cell?

What is mRNA

200

What molecule is produced before PCR in RT-PCR

What is cDNA

200

What is RNAi used for?

What is temporarily silencing gene expression

300

What technique uses probes to visualize gene expression in intact tissues or embryos?

What in situ hybridization

300

What process amplifies DNA after cDNA is made?

What is PCR

300

What does SNP stand for?

What is single-nucleotide polymorphism

400

What label is attached to probes so they can be seen?

What is a Fluorescent tag

400

What does qRT-PCR measure?

What is mRNA levels

400

What does a gene drive do?

Spread new alleles in a population

500

What property of nucleic acids allows probes to bind 

What is complementary base pairing

500

What type of data does gel electrophoresis show after RT-PCR

What is DNA bands

500

How are SNPs used to study diseases?

What are genetic markers