Background
In C. elegans, this is the number of plexin genes.
What is two?
A valuable method to precisely disconnect single neurites in intact animals without causing much collateral damage
What is laser ablation/microsurgery?
According to the figures, PLX-1 expression in C. elegans is predominantly in this tissue category.
What is non-neuronal tissue?
The overall effect of plexin knockouts on baseline neuromorphogenesis in intact animals.
What is no effect?
This is the rationale for choosing C. elegans for dissecting semaphorin/plexin function.
What is well-established model with a constrained number of plexins and semaphorins?
The only semaphorin that is secreted in C. elegans.
What is mab-20?
Knockout animals would express GFP here.
What is the nervous system?
Among the null mutants tested, this plexin mutant showed the highest axon reconnection in vivo.
What is plx-2?
The primary result of plexin knockout mutants in neural regeneration.
What is increased regrowth and reconnection?
The year this article was published?
What is 2022?
The plexin that binds to membrane-bound semaphorins.
What is PLX-1?
The specific class of neurons whose axons were targeted for microsurgery and behavioral analysis in this study.
What are motoneurons?
This null mutant increased swimming speed when compared to WT C. elegans.
What is mab-20?
The hypothesized signaling direction that could allow PLX-1 to directly affect motor neuron responses.
What is reverse signaling (from plexins to semaphorins)?
The journal this article was published in.
What is Frontiers in Cell and Developmental Biology?
In addition to plexins, the other class of transmembrane proteins that can assist with Semaphorin binding.
What is a neuropilin co-receptor?
The method used to verify knockout animals expressed their specific strain upon their arrival, after crosses, and after the study.
What is polymerase chain reaction (PCR)?
The unit of speed used in this study.
What is μm/s?
The proposed explanation for faster swimming in mab-20 mutants.
What are downstream effects on tissue structure and gene expression?
The supplementary figure that demonstrates the effect of semaphorin knockouts on regrowth and reconnection?
What is there is no supplementary data?
Not a transmembrane domain, this special link helps bind membrane-bound semaphorins.The name of the bond that holds semaphorins in the transmembrane domain.
What is a glycosylphosphatidylinositol (GPI) link?
This specific experimental control group underwent all handling steps—including cooling, immobilization, and mounting—but was not exposed to the laser.
What is the sham-surgery group?
In Figure 4, the genotype(s) that recovered normal locomotion in 24 hours, when compared to sham locomotion.
What are all genotypes?
Secondary messengers the authors would like to study for their involvement in the semaphorin signaling pathway.
What are calcium and cAMP?
Your favorite part of reading this article.
What is {___}?