PWS Basics
This genetic disorder is characterized by hypotonia, hyperphagia, and obesity.
What is Prader-Willi Syndrome (PWS)?
This process causes one parental allele to be silenced.
What is genomic imprinting?
This gene-editing tool was used to knock out ZNF274.
What is CRISPR/Cas9?
After ZNF274 knockout, ZNF274 binding does this.
What is decrease?
PWS is caused by the loss of gene expression from this parent.
What is the paternal chromosome?
In PWS, maternal genes are present but ________.
What is silenced?
This method was used to measure gene expression levels.
What is qRT-PCR?
After ZNF274 knockout, H3K9me3 levels do this.
What is decrease?
This specific chromosome region is affected in PWS.
What is chromosome 15 (q11-q13)?
What is H3K9me3?
This technique measures protein-DNA interactions.
What is ChIP (Chromatin Immunoprecipitation)?
These types of genes were reactivated after ZNF274 KO.
What are maternal PWS genes?
This condition occurs when both chromosome copies come from the mother.
What is maternal uniparental disomy (UPD)?
This protein is responsible for adding repressive marks in PWS.
What is ZNF274?
These cells were used and then differentiated into neurons.
What are induced pluripotent stem cells (iPSCs)?
Gene reactivation was strongest in this cell type.
What are neurons?
This early-life symptom of PWS is characterized by low muscle tone.
What is neonatal hypotonia?
This region controls imprinting and remains methylated even after KO.
What is the PWS imprinting center (PWS-IC)?
This cell stage comes before mature neurons in differentiation.
What are neural progenitor cells (NPCs)?
These genes were NOT rescued after ZNF274 KO (name one).
What are SNRPN exon 1; NDN?