Gill's Hematoxylin
Safety
Special Stains
Slides, mounting media, and coverglass
Processing
100

The base of the dye

Water

100

What does RACE stand for?

Respond/Rescue, Activate (Alert), Contain/confine, Escape/Extinguish

100

Stains mycobacterium in TB

AFB

100

T/F - Mounting media should be placed on the slide, then the coverslip lowered down on top. 

False

100

What processing error happened here?

Water in the xylene

200

Increases the solubility. The "E"

Ethylene glycol

200

It is important that this chemical used in the cytology laboratory is not discarded down the sink.

Xylene

200

Oil Red O is used to stain lipid. This stain is another alternative. 

Sudan Black

200

Standard size of cytology slides

3x1 in.

200

Thick specimens can lead to poor leveling because this stain did not have time to fully penetrate all of the cells.

Fast green / light green

300

The oxidizer, "S"

Sodium iodate

300

In regards to an ABC fire extinguisher, the C stands for this type of fire.

Electrical (Current)

300

Stains glycogen

PAS

300

The recommended thickness for cytologic preparations.

.13-.17mm / No. 1

300

A bloody specimen can be treated for blood lysing before processing with saponin or after processing by emersion fixing the sample in this solution.

Carnoy's solution

400
The role of ammonium aluminum sulfate

The mordant

400

What is this symbol?

NFPA

400

The closest stain to the Pap stain. Dr. Papanicolaou was working with this as well.

Trichrome

400

Three methods for removing a coverslip (besides just forcing it off with a blade). 

Heat, Cold, Xylene

400

Saccomanno fixative is prepared by adding this to 50% ethanol.

2% polyethylene glycol (or just Carbowax)

500

Lowers pH. It's also used separately as a blood lysing agent.

Glacial acetic acid

500

What hazard does this symbol represent?

Oxidizing agent

500

Stains for melanin and argentaffin granules (historic)

Fontana-Masson

500

The ideal refractive index for cytology

1.523 (1.51-1.53, 1.5 acceptable)

500

Various concentrations of alcohol can remove the OG and EA stains. What is used to remove the hematoxylin?

HCl

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