The Animal Research Environment
The Animal Welfare Act is a federal mandated law that established legal standards for laboratory animal care and use. Who administers the AWA?
United States Department of Agriculture (USDA)
What is the common term for the policy that most institutions have to protect an individual reporting instance of animal abuse or neglect?
Whistle-blower policy
What is the primary source of the standards and guidelines for laboratory animal care and use in the United States?
The Guide for the Care and Use of Laboratory Animals
What are the 3R’s?
1. Replacement
2. Refinement
3. Reduction
What does CDC stand for?
Center for Disease Control
When working with infectious agents
Animal Welfare Regulations (AWR) define the roles of the different individuals in the animal facility. Who administers the AWR?
Animal & Plant Health Inspection Service (APHIS)
What document must an investigator submit for approval by the IACUC for each research study involving laboratory animals?
Animal use protocol or animal protocol
Which species does the PHS Policy on Humane Care and Use of Laboratory Animals cover?
All live vertebrates used or intended for use in research, research training, experimentation, biological testing, or for related purposes. This includes ectothermic vertebrates such as fish, amphibians, and reptiles.
Name the three key factors for personal protection from radiation.
1. Shielding
2. Time
3. Distance
What is the term for an animal disease that can be transmitted from animals to humans?
Zoonosis
Common diseases:Rabies, Tuberculosis, Toxoplasmosis, Q-Fever, and B-Virus
The AWA governs the use of warm-blooded vertebrates (live or dead), except for these species:
1. Birds, rats, and mice bred for research
2. Farm animals used for use as food, fiber, or for agricultural research.
Good Laboratory Practices (GLP's) apply to animal and non-animal research and safety studies funded or reviewed by the Food and Drug Administration and the Environment Protection Agency.
Who ensures compliance with the GLP's?
The Quality Assurance (QA) Unit
The PHS Policy on Humane Care and Use of Laboratory Animals applies to federally funded research projects. For an institution to comply with PHS Policy, what document must be signed and submitted?
The Animal Welfare Assurance of Compliance Statement
Ear protectors should be worn in areas such as cage wash where the noise level measures at or above _____?
85 Decibels
Whose standards specifically address the storage and handling of radioactive materials in the facility?
Nuclear Regulatory Commission (NRC)
How often do USDA-APHIS inspectors perform unannounced visits?
At least once a year
According to the AWR, at least 3 members are required on an IACUC (Institutional Animal Care and Use Committee), who are they?
1. The Chair (chairperson)
2. Veterinarian (with training or experience in lab animal medicine)
3. Non-affiliate (public) member
What agency is responsible for enforcing the Public Health Service Policy on Humane Care and Use of Laboratory Animals (PHS Policy)?
Office of Laboratory Animal Welfare (OLAW)
The Animal Welfare Assurance of Compliance Statement should be signed and submitted to OLAW.
Name three types of indicators used to ensure that an autoclave cycle was effective.
1. Mechanical
2. Chemical
3. Biological
What document should be available for all chemicals used within the animal facility?
Safety Data Sheets (SDS or previously the MSDS)
Provides information on toxicity, potential side effects, and recommended treatment after potential exposure.
How often must an IACUC perform inspections of the facility?
Every 6 months
According to the PHS Policy, at least 5 members are required on an IACUC (Institutional Animal Care and Use Committee), what are the 4 must-haves?
1. The Scientist using animals
2. Veterinarian (with training or experience and has program authority)
3. Non-scientist
4. Unaffiliated member
Who has the authority to sign and submit the Animal Welfare Assurance of Compliance that the animal research program meets all relevant regulations and standards?
Institutional Official
What law requires employers to provide a workplace free from hazards that can cause serious injury or death?
Occupational Safety and Health Act (OSHA)
What does BMBL stand for?
Biosafety in Microbiological and Biomedical Laboratories.
Published by the CDC, providing biosafety guidelines for protecting workers and preventing exposures in biological laboratories.