Technical skills & application/biotechnology skills
A solution has an absorbance of 0.5 and a concentration of 0.2 M. What would the absorbance be if the concentration of the solution is doubled?
1.0
In the U.S., CGMPs are regulated by which agency?
fda
Why are enzymes necessary in living organisms?
Enzymes increase the rate of chemical reactions which would otherwise occur too slowly to sustain life.
What is the purpose of the tare function on an electronic balance?
To reset the display to zero
How does replication contribute to the reliability of a study?
By providing multiple data points that can confirm consistency and repeatability of the findings.
Which type of chromatography is primarily used for the separation of proteins based on their charge?
Affinity
Which biotechnology role is responsible for: Participates in the production of commercial quantities of a product
Manufacturing
Where does translation take place in prokaryotes?
ribosomes
A technician is testing the purity of a DNA sample using a spectrophotometer. He gets a 260 nm reading of 0.51 au, and a 280 nm reading of 0.65 au. Recalling that the preferred range for the value is 1.8-2.0, is the purity of the sample acceptable?
No
Explain independent and dependent variables
Independent: intentionally manipulated
dependent: measured variable
When preparing buffers or media, when should a technician adjust for pH?
After the solution has been brought to its final desired volume, to ensure accurate pH adjustment.
CGMP is an abbreviation used in the pharmaceutical and biopharmaceutical industry, and stands for:
Current Good Manufacturing Practices
Complimentary base pairing between two DNA strands is due to the formation of:
hydrogen bonds
A sample has a known pH value of 4.5, but the pH meter reads 5.2. What should be done to ensure accurate measurements?
Rinse the electrode with tap water and measure again.
a law describes what nature does under certain conditions, and will predict what will happen as long as those conditions are met. A theory explains how nature works.
A technician is performing an assay with a 30 minute incubation step. If the technician begins the incubation at 14:42, at what time will the incubation step end?
15:12
While performing an assay, a technician neglects to label the samples. Rather than repeating the assay, the technician records the results at random. A coworker observes the incident. Which of the following is the appropriate action for the coworker to take?
report the error to the supervisor
When DNA replicates, each original strand of DNA serves as a template for a new strand. What type of replication is this?
Semi-conservative
True or False: Empty glassware can be sterilized by autoclaving on a dry cycle at 21°C and 5-10 PSI.
False: Empty glassware can be sterilized by autoclaving on a dry cycle at 121°C and 15-20 PSI.
What is the purpose of calculating the p-value in statistical analysis?
The p-value helps assess whether the results are statistically significant, indicating whether the null hypothesis should be rejected / To determine the probability that the observed results occurred by chance.
What is one major advantage of using flow cytometry over traditional microscopy techniques?
It allows for the simultaneous analysis of multiple parameters on thousands of cells in a short period of time.
A red triangle with a black exclamation point represents what warning symbol
Harmful/irritant
E. coli migrating towards a high concentration of glucose is known as ____taxis
chemotaxis
Why is it important to avoid using an open flame inside a BSC?
Flames can disrupt airflow inside the BSC.
Why is it important to standardize or normalize data before applying principal component analysis (PCA)?
Standardizing data ensures that each variable is on the same scale, allowing PCA to identify meaningful patterns rather than being influenced by the scale of the variables.