Math
How many sig figs are in 873,010?
5
You are a part of a manufacturing team creating a small molecule drug. Your role is to ensure all documentation is done correctly and complete any necessary FDA applications. What is your position?
QA
What is NOT considered an advancement in PCR?
Use of DNA gel electrophoresis
What should you NOT use to destroy DNA?
Alcohol
What type of ELISA uses two primary antibodies?
Sandwich
What is 0.008573 in scientific notation?
8.57 x 10^-3
What type of document does QC use?
Test record
How are paternity tests completed?
DNA gel electrophoresis
What discovery allowed for the beginning of recombinant DNA technology?
How is primer temperature determined?
The % of C's & G's
You need to make 605mL of a laboratory cleaning solution with a 2:43 dilution. How much disinfectant in mL do you need? Record your answer to one decimal place
28.1
What type of document does Manufacturing use?
SOP and batch record
What are the parts of the cell theory?
All life is made of one or more cells
Cells only arise from pre-existing cells
Cells are the smallest form of life
List 3 vectors you can use to get DNA into a cell
Plasmid
Virus
Lipid/liposome
What are the steps of PCR with description and temperatures?
Denature - 95C - high heat breaks H bonds separating DNA
Anneal - 50-70C - primer attaches to the gene of interest
Extend - 72C - Taq polymerase adds dNTPS and lengthens the strand
You have a 2.0mg/mL BSA solution. You add 110uL to a tube with diluent in it for a final volume of 275uL. What is the dilution factor?
22/55
Why are monoclonal antibodies used over polyclonal antibodies for certain diagnostic tests?
Mabs recognize only one epitope
A plasmid has 4 restriction sites for HaeIII. If digested with HaeIV how many fragments will result?
1
A technician performing an ELISA uses a primary antibody with a chicken IgG2a isotype to analyze a human blood samples for tuberculosis. What is the name of the primary antibody?
Chicken anti-tuberculosis
What makes a good primer?
Not too long (20ish BP)
Not a lot of repeats
Antiparallel
How many g of NaOH are need to make 0.029mL of a 975ug/uL solution ? Record your answer to THREE decimal places
0.028
Put these steps in order:
1. Research & Development
2. Safety & Efficacy testing
3. Large scale manufacturing
4. Process development
5. Small scale manufacturing
1, 4, 5, 2, 3
Put these steps in order:
1. Sales and Marketing
2. Clinical Trials
3. Research and Development
4. Identify a product
5. Manufacturing
4, 3, 2, 5, 1
What are the steps of mab production?
Immunize specimen
Immunize again to boost response
Collect serum
Isolate B cells
Fuse with tumor cells
Harvest hybridoma
What are the steps of genetic engineering?
Isolate gene
Paste gene into vector
Transform bacteria with recombinant vector
Grow on selective media
Purify gene product