Pipetting
Math
Health and Safety
Bacteria
Experiments
100
This type of pipet is used to measure small volumes (microliters)
What is a micropipette?
100
You guys learned about C1V1s. What does the C and V stand for
What is concentration and volume
100
These 3 articles of PPE are ALWAYSS required in the lab
Gloves, Safety glasses, Lab coat
100
We've been working with this bacteria.
E.coli
100
What was your favorite experiment and why?
NO WRONG ANSWERS heheheeh
200
This pipet is used to measure larger volumes (1-50 mL)
What is a serological?
200
I have 100% isopropanol and want to make 2.5 liters of 70%, how much of the 100% do I have to use.
1.75 L of 100%, QS to 2
200
You see someone spill a chemical, what should you do first?
Let your supervisors and other know right away!
200
This is a circular piece of DNA used by bacteria to swap traits
Plasmid
200
DNA has what charge
Negative
300
You go to the first stop on a micropipette when doing this
What is aspirating?
300
I have 5*10^8 HEK cells in my flask. How many flasks can I make at a concentration of 1*10^5
What is 5000 flasks
300
What does this mean?????????????????????????
What is an irritant?
300
What is the collection of bacteria called
What is a colony?
300
Large pieces of DNA are at the _________ of the gel and small DNA pieces are at the _______ of the gel
What is top and bottom
400
Susan want to pipette 7.4 mLs. She is stuck! Explain to her how you would do this, include what tools you would use and what tube you would choose
Oh susan! Let me help. You could do two things, use a serological to measure 7.4, checking at eye level. Or you could measure 7 with a serological and 400 uL with a P1000. Put it into a 15 mL conical tube
400
My Tris buffer stock is at 5M, I want 500 mLs of 40mM of tris buffer. How much buffer stock do I need?
What is 4 mLs of stock
400
OH MY GOD! We found NAOH in the warehouse that we need to neutralize it! It's at a pH of 14, how do you neutralize this
omg let me help. With a friend, you should set up a pH probe in a huge vat of water and neutralize SLOWLY with a weak acid
400
This is the process of putting a plasmid into a cell
Transformation
400
What color are lactose intolerant e.coli
White
500
Name TWO common ways to break your pipette
Putting it upside down and setting out of range
500
You want to make 6L of a 5M salt solution. The FW of salt is 58.44. How much salt do I need?1
1,753.2 grams
500
What's wrong with this picture??!!!
No shoes, food, unlabeled beakers mess, no gloves, no goggles, fire eefhowigoewb not in glass
500
How does CRISPR work?
Cas9 cuts DNA at a specific spot using guide RNA. DNA gets repaired by being induced and with guide DNA.
500
Describe how transformation uses temperature (cold, hot, cold) to get the plasmid into e.coli
The cold CaCl makes the plasmid positive and thus attracts to the negative DNA in the cell. The heat make little holes in the cell membrane so the plasmid can fall into the cell. The cold locks the plasmid into the cell so it doesn't fall out