5'TACGAGGTACCCGATGTA3'
The amino acid chain that this coding strand codes for.
What is Met-Glu-His?
100
Uses both forward and reverse primers, all 4 dNTPs, Taq polymerase, buffer, and a DNA template
What is PCR?
100
List steps of mitosis and ploidy for each
What is Prophase (2N, 4C)
Metaphase (2N, 4C)
Anaphase (4N, 4C)
Telophase (4N, 4C)
100
Define complementation group
What is group of mutants that FAIL to complement one another
100
Materials used in sequencing
What is all 4 dNTPs, 1 fluorescent ddNTP per tube, 1 primer, DNA polymerase, buffer, polyacrylamide gel?
200
This is how we format our nucleotide sequence in 4Peaks to be compatible with CLC sequence viewer
What is reverse one strand so that both strands are going in the same direction. (so reverse 16R OR reverse 16F)
200
This is why we clean PCR products before sequencing
What is eliminate forward and reverse primers?
200
terminology for only having one allele
What is hemizygous?
200
Definition of phototroph
What is an organism that can live on minimal media?
200
12. The 3 kb band in the DNA ladder and your 5 μl of your PCR product have the same fluorescence intensity on your gel. Your PCR product is 1.5 kb in length. You can conclude that
A)The 3kb band has 2X the number of DNA molecules as 5μl of your PCR product.
B)The 3kb band has 1⁄2 the number of DNA molecules as 5μl of your PCR product.
C) The 3 kb band has 4X the number of DNA molecules as 10 μl of your PCR product.
D) The 3 kb band has the same number of DNA molecules as 5 μl of your PCR sample.
E) A and C.
What is B?
300
P.Phe459Tyr means
What is a change of 459th amino acid from N terminus from Phe to Tyr?
300
This is what goes into the negative control of PCR
What is same as PCR but water instead of template?
300
Genetically linked alleles violate which of Mendel's laws?
What is Mendel's 2nd Law?
300
Describe differenced between SD and YPD in terms of lab 6
What is SD:synthetic defined medium
minimal media lacks histidine
YPD: complete medium with histidine
300
A certain piece of DNA is about 4 billion (4 x 109) base pairs long, and its bases are more or less randomly distributed. How long should a primer be so that, statistically speaking, it will only bind once to this DNA (ignore partial
hybridizations)?
A. 10bases
B. 14 bases
C. 15 bases
D. 16bases
E. 18 bases
What is D?
400
A type of substitution that causes no change in amino acids
What is silent mutation?
400
Purpose of doing gel electrophoresis (in context of lab 6)
What is find detectible DNA band
check if band is right size
find concentration of PCR product
400
State law of segregation
What is allele pairs separate or segregate during gamete formation randomly, with one randomly going to each gamete?
400
What is the key requirement about the mutation for complementation to work?
What is recessive mutation?
400
Type of gel that allows resolution to one nucleotide which allows use in Sanger sequencing
What is polyacrylamide gel?
500
homologs resulting from gene duplication in a species
What is a paralog?
500
Do we need a buffer system in gel electrophoresis? Why/why not
What is no, it's already in gel.
500
Draw prophase vs. interphase in onion root tips
What is see study guide
500
What is his?
What is an essential amino acid?
500
What is 16S and what is its purpose?
What is rRNA gene used bc all species have ribosomes and rRNA bases with enzymatic function highly conserved (makes convenient target), while intervening bases without catalytic functions widely divergent