PCR
The second of the main three steps of PCR
What is Annealing?
The proteins chromatin wraps around
What are histones?
Used for both RNA and DNA tracking in an organism
What is a nucleic acid probe?
Not present in Bacteria, Sometimes Present in Archaea, and very present in Eukarya
What are introns?
As opposed to in vitro
What is in vivo?
Buffer, Template, Taq Polymerase, free nucleotides, and Primers
What are components of PCR?
The process of turning a "gene" on by revealing the DNA wrapped around a certain protein
What is acetylation?
The gel used for protein detection
What is a Western?
Ratio between base pairs and number of genes
What is gene density?
Used to cut both the plasmid and GOI so that they match when introduced to one another
What is a restriction enzyme?
The location that taq polymerase was discovered and utilized in PCR for its heat stability
What is the Yellowstone?
The process that both methylation and acylation help regulate in terms of gene expression
What is transcription?
In contrast to knowing what molecules are present, this analysis tells us where a certain gene is expressed
What is in situ hybridization?
Consisting of about 3.2 billion base pairs and about 24,000 genes
What is the Human Genome?
DNA molecule (often a bacteria or plasmid) used to carry a DNA fragment as part of cloning
What is a cloning vector?
The section of DNA template that must be known before PCR can be done
What is the flanking region?
These accumulate over a lifetime and most are reset during gamete formation
What are epigenetic tags?
Used to compare patterns of gene expression in different tissues, at different times, or under different conditions
What is a microarray assay?
Transposable elements that use an RNA intermediate
What are retrotransposons?
The largest genomic library with a capability of more that 2000 kb
What is HAC?
The reason primers must face one another
What is the 3' end?
Works in conjunction with the molecular mechanism of epigenetics
What is piwi RNA?
useful for comparing amounts of specific mRNAs in several samples at the same time
What is Reverse transcriptase-polymerase chain reaction (RT-PCR)?
Queen who discovered transposable elements, whose work had many haters
NOBEL PRIZE WINNNNER Dr. Barbara McClintock
The typical purpose of gene cloning
What is protein harvesting?