Trafficking
Gene Regulation
Post-Transcriptional Regulation
DNA Mutation & Repair
Human Gene Therapy
100

The chloroplast has this many membranes.

What is 3?

100

The Acetylation of lysine's on histone tails impacts chromatin packing. This is an example of what type of gene regulation?

What is post-translational modification? 

100

A protein will be tagged with this be degraded.

What is ubiquitin?

100

An individual has a mutation in their somatic cells that causes their hair to be pink. Will this individual's children express the same mutation?

What is no, the mutation is not in their germ cells.

100

A couple participating in IVF wants to find out what is their chances of having a child with a certain disease. The procedure should be done to find out these chances.

What is pre-implantation screening?

200

This signal on this terminus of the protein will cause translation to halt and the protein to be translocated to the ER.

What is the Hydrophobic N-terminal signal sequence?

200

In a fruit fly there is an enhancer needed to be expressed to have wings. During splicing in the germ cells the exon that contains the wing enhancer is spliced out. The offspring of these flies will appear.

What is wingless?
200

The insulin receptor comes from the same mRNA strand. Insulin receptors in the brain have a higher affinity for glucose than insulin receptors in the liver. This RNA processing event is responsible for this.

What is alternative splicing?

200

A thymine dimer can be repaired through this process.

What is nucleotide excision repair?

200

An individual is getting treatment for lung disease through a gene therapy. This individual has to get treatment every three months. This individual is getting what type of delivery method?

What is AAV?

300

The cytoskeleton is a filamentous network that contains these proteins.

What is actin filaments (microfilaments), microtubules, and intermediate filaments?

300

This is the movement of mRNA and regulatory transcription factors through nuclear pores.

What is mRNA leaves the nucleus through the pores and regulatory transcription factors enter in through the pores?

300

 An individual is found to have very little vision due to loss of function mutation of the Sight gene, which is required for the production of siRNAs. What mechanism can explain the loss of vision?

What is retrotransposons have become active and inserted themselves into new places of the genome?

300

This type of mutation causes a rabbit's fur to grow twice as fast.

What is gain of function?

300

AAV is used to deliver a gene to make an individual resistant to HIV to germ cells. Will the individuals born from these germ cells be HIV resistant?

What is No

400
A cell has a dysfunctional mitochondria, resulting in no ATP being produced. This will do what to the movement of motor proteins.

What is no movement will occur?

400

A cancer causes too much cell divisions. A protein called CAM is involved in this cancer. Myeloma is a type of cancer that results from too much cell division. In enhancers of genes that can cause cell proliferation, CAM has been shown to be able to open up heterochromatin. CAM recruits what kind of factor?

What is CAM has binding affinity for HATs?

400

When estrogen binds to the estrogen receptor, the estrogen receptor moves to the nucleus and transcription occurs. The estrogen receptor is what type of protein?

What is a regulatory transcription factor?

400
DNA pol makes very few mistakes due to this ability.

What is proof reading?

400

An individual has been gotten RTV treatments for a blood issue. Will the individual children express this treatment?

What is no

500

A transmembrane insulin receptor binds directly to insulin through the part of the receptor that is located in the extracellular space outside of the plasma membrane. The insulin receptor is endocytosed. The part of the insulin receptor that binds to insulin now faces this direction in the an endosome.

What is the part of the insulin receptor that binds to insulin would now face the lumen of the endosome

500

A researcher has decided that they want to make humans express fly wings. The researcher adds in a fly wing promoter and a fly wing enhancer. The individual does not express fly wings. What must be missing from the individual?

What is the regulatory proteins?

500

Two different splice isoforms of the gene CAT are present in cells. Researchers studying these splice isoforms apply a drug to cells that stops any new transcription and then monitor newly translated CAT protein. They find that the two isoforms generate identical CAT proteins. Cells carrying Isoform 1 continue to translate new CAT protein for a long time after transcription is stopped, while cells carrying Isoform 2 translate new CAT protein for only a short time after transcription is stopped. What could cause this difference in translation times?

What is difference between mRNA stability? 

500

When reading a PCR table, the strands of this length will travel further down the grid.

What is shorter strands?

500

A disease results from a deletion of 7 nucleotides in a particular gene, resulting in a frameshift and an early stop codon downstream. These components are necessary to use CRISPR to repair this mutation.

What is DNA donor template for repair, CAS9 protein, and guide RNA?