Show:
Amino Acids
Secondary Structure Facts
Separation Techniques
Protein Sequencing
Nobel laureates
100

These two amino acids are aromatic and are cut by the enzyme chymotrypsin and have a UV absorbance around 280nm. 

What is F Y W  (Phe, Tyr, Trp)



100

What is the pitch of an alpha helix?

5.4 Angstrums 

100

What charge is a CM column? 

Negatively charged! (this means that negative and 0 will elute first TOGETHER!!) 

100
What can NOT be the next AA for Chymotrypsin, Trypsinogen, or elastase?  

Proline 

100
This man won the Nobel Prize for sequencing bovine insulin and for his work in DNA sequencing! 

Frederick Sanger: He won TWO Nobel prizes. 

200
The pka 1 and pka 2 of every amino acid is important for degerming the amino acid from this type of graph? 

Titration curve! 

200

What are the phi and psi angles of Beta Sheets? 

approximately +180 and -180 respectively. 

200

What will come out first in a size exclusion column?  

The largest elute first 
200

What is an N-Terminal reagent used in Edman Degradation?  

PITC (What conditions are needed acid/basic and for which steps?) 

200

What elution technique did we talk about that won a Nobel prize.  

Cryo-electron microscopy

300

This amino acid has a side chain pka of 8.5 and is one of two amino acids with the element sulfer. 

Cystine 

300

What bond is used to determine  heavy metal posing and is found in tertiary but not quaternary structures? 

Disulfide Bonds 

300

What two separation techniques will have a log relationship? 

Gel Filtration Chromatography and SDS Page 

300

What does an invariant residue mean? 

The AA does not change at All!! 

300

These two men are founders of structural biology and discovered complex secondary structures. 

Linus Pauling and Robert Corey

400

What is the net charge of amino acid W at a pH of 10.5? 

NC = -1 (the COO- and the NH20) 

400

What is the repeating structure of silk Fibroin? 

-Gly-Ser-Gly-Ala-Gly-Ala repeating!! 

400

What do the following blotting techniques do? 

Western? 

Southern? 

Western: Moves proteins from a gel to a membrane.

Southern: Moves DNA from a gel to a membrane.  

400
What are the two ways to cleave Disulfide bonds? 

1. React with excess thol and cap with Iodoacetic acid

2. Oxidize with performic acid 

400

Won the Nobel Prize for discovering prions as a new form of biological infection. 

Stanley Prusiner

500
The PI of this polypeptide: 


HYGIVK

PI = 9.5 

500

What are the primary bonding interactions for the following structures?

Primary: ? 

Secondary: ? 

Tertiary: ? 

Quaternary: ? 

Primary: Covalent Bonds 

Secondary: H-Bonds 

Tertiary: Hydrophobic effect 

Quaternary: Hydrophobic effect (No disulfide bonds) 

500

What comes out first in a REVERSE phase HPLC? 

The most hydrophilic (or most polar molecule) Need to know the polarity of the AAs!!! 

500

What do the following things cut after? 

Chymotrypsin? 

Elastase? 

Trypsinogen? 

Cyanogen Bromide? 

Chymotrypsin: F Y W 

Elastase: G A S V 

Trypsinogen: K R 

Cyanogen Bromide: M

500

Won the Nobel prize for denaturation and renaturation of ribonuclease A (RNase A). 

Christian Anfinsen