Amino Acids
Which form are amino acids found in the human body?
L conformation
What is the Henderson Hasslebach equation?
pH = pKa + log [A-]/[HA]
Primary: amino acid sequence
Secondary: alpha helices and beta sheets
Tertiary: Folding of protein, uses R group
Quaternary: multiple proteins joined together
When performing Sangor sequencing and Edmun degradation which end of the protein are you trying to determine?
N terminus
What is a zwitterion?
Has both a negative and positive charge within the same ion.
Describe what will happen to the hydrogen if the pH is greater than the pKa.
The hydrogen will be removed.
What type of bond is a disulfide bond and what can be used to break them?
Covalent bond that can be broke with chemicals (ex: perform acid)
What is the purpose of SDS gels?
They make the proteins all into the same shape and charge so they are only separated by size.
Name 5 essential amino acids.
Valine, Leucine, Isoleucine, Methionine, Threonine, Phenylalanine, Tryptophan, Lysine, Arginine, and Histidine.
What is the concentration of water in pure water?
55.5 M
Which aino acids are not usually found in alpha helices and why?
Proline because it is too rigid
Glycine because it is too loose
Of the three methods of purifying proteins, which is the hardest and why?
Affinity is the hardest because you have to know what your protein is, so you can know what it will bind to.
What type of reaction between two amino acids forms a peptide bond?
Dehydration or synthesis reaction
Where does a buffer exhibit it's maximum buffering capacity?
pH = pKa
Which conformation of beta pleated sheets is stronger?
Anti-parallel
Do agarose gels run horizontally or vertically?
Horizontally
Name one amino acid that can be phosphorylated?
Serine, Threonine, and Tyrosine. Any amino acid that has an -OH.
Calculate the pH of 0.20 M HC2H3O2 and 0.50 M C2H3O2- that has a Kb of 5.56 x 10-10.
5.1
Where are R groups found in alpha helices(outside or inside)?
Outside
Where does Trypsin cleave proteins?
A the C-terminal end of Lysine and Arginine