Biochemistry
Gene Regulation and Expression
100

What are the three most common types of RNA?

mRNA

tRNA

rRNA


100

What is the most common form of DNA?

Right-handed (B-DNA)

200

What are the two eukaryotic ribosomal subunits and what is the ribosome size when they come together?

40S, 60S, and 80S

200

What is a common problem that restrictions may face causing them to be unable to cut DNA?

Methylations

300

What are the three common disaccharide sugars and their components?

Sucrose (glucose + fructose)

Lactose (glucose + galactose)

Maltose (glucose + glucose) 

300

What enzyme is key in blue-white screening and what does it do?

β-galactosidase, which cleaves lactose into glucose and galactose.

400

In glycolysis, what is the rate-limiting step (numerical value), and what is this reaction's substrate, enzyme, and product?

Step 3, fructose-6-phosphate, phosphofructokinase, 1,6-bisphosphate.

400

What are self-splicing RNA's called?

Ribozymes

500

What chemical is used to synthesize peptide chains (specifically the peptide bond) to make the reaction energetically favourable?  

DCCD (dicyclohexylcarbodiimide)

500

Which is known as the "bridge" snRNP in the splicesome cycle.

U5