Osmosis
What vegetable are we using to perform the osmosis experiment?
Potatoes
What is the substrate in the enzyme lab?
Azocasein
What is the highest volume of a P1000 pipette?
1000ul
What is the range of p-value to be considered "statistically significant"?
less than or equal to .05
In an osmosis experiment, how do you determine if osmosis has occurred in potato cores?
By measuring the change in weight of the potato cores.
What do we add to stop the enzyme reaction?
TCA
What machine in lab do we use to produce a pellet and supernatant?
Centrifuge
Name the calculation that measures the average variability in your dataset
standard deviation
What role does high temperature play in the rate of osmosis?
Higher temperatures increase the rate of osmosis.
What does a high absorbance reading tell you in the enzyme experiment?
The enzyme activity was high
What do we do to the spectrophotometer before we start measuring our samples?
Blank, usually with 1000ul of water
You run a t-test comparing a control and experimental group and get a p-value of 0.50. What does this tell you?
The two groups are not significantly different.
An animal cell placed in a hypertonic solution will?
Loss water and shrink
For the enzyme experiment, if your independent variable is temperature, what variables stay the same?
salinity, pH
What unit (that we use in class) does the scale measure?
grams (g)
How would you make 1000 μl of a 1:5 dilution of dye in water?
200ul dye + 800ul water
What direction does water move if you place a cell containing 2M sucrose into a large beaker containing 1M sucrose?
Water moves into the cell
What bonds are impacted when trypsin is exposed to a drop of pH from pH 8 to pH 5?
Hydrogen and ionic bond
What is the proper way to pipette a sample liquid into a tube?
Set the pipette to desired volume, attach the tip, go to first stop, pipette the sample, go the second stop dispensing the liquid into your tube
How would you make 1200ul of a 1:20 dilution of dye in water?
60 ul dye + 1140 ul water