Hemolysis
General Media
Lactose Fermentation
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Other
100
A clear zone of hemolysis indicated a __________hemolytic organisms.
beta
100
The essential nutrients present in media can be abbreviated CHNOPS, what does the C stand for?
carbon
100
This media has a purple tint to it and is selective for Gram negatives.
MacConkey
100
What kind of hemolysis is this?
gamma
100
This media is best for growing fastidious organisms.
Chocolate agar
200
CNA can show hemolytic patterns and is also selective for which organisms?
Gram positive
200
What is the jelly-like component present in culture media?
agar
200
This media turns yellow when a lactose fermenter grows on it.
Hektoen enteric
200
What kind of hemolysis is this?
beta
200
This media has so many antibiotics in it that only organisms like N. gonorrheae can grown on it.
MTM
300
If an organism has a green color surrounding the colonies on a blood agar plate what kind of hemolysis would this be?
Alpha hemolysis
300
Define general media.
Media that has all the essential nutrients that will not inhibit growth.
300
S. epidermidis turns tan on MacConkey agar because it doesn't ferment lactose. True or False?
False.
300
What are two facts about the metabolism of this organisms?
NLF and H2S+
300
We plated P. mirabilis and P. aeruginosa on HE media, which one makes black colonies?
P. mirabilis
400
In which kind of hemolysis are bred blood cells completely destroyed?
Beta hemolysis
400
Why do we use agar instead of gelatin or potato for media plates?
Agar is not degraded by microorganisms and stays solid at room temperature.
400
E. coli has pink colonies on MacConkey agar and yellow colonies on Hektoen Enteric because it is a strong lactose fermenter. True or False?
True
400
Give the kind of media and a fact about the metabolism of this organism.
MacConkey NLF
400
What color would P. aeruginosa colonies be on a MacConkey plate?
tan
500
What is the virulence factor that allows an organism to perform hemolysis?
Hemolysins
500
Who came up with the idea to use agar?
Fannie Hesse
500
If you were to classify Hektoen enteric media using the terms general, selective, or differential which would apply?
Selective for Gram negatives and Differential for lactose fermentation and H2S.
500
Give the name of the media shown here and 2 facts about the metabolism of this organism.
HE NLF H2S-
500
Does E. coli grown on CNA plates?
no