Cell Differentiation
Organs are composed of this many tissues
What is two or more
The cellular ability to "become anything"
What is pluripotency
I am a cell that was isolated from tissue, then grown and subcultured in growth media. What am I?
What is secondary cell culture
The domain of an intracellular signaling protein that recognizes phosphorylated tyrosines
What is SH2 domain
Patterns of light that reveal size and granularity as cells pass through a flow cytometer
What is forward and side scatter
The basis of specialized cell type formation (differentiation)
What is differential gene expression
A division event producing two different kinds of cells
What is asymmetric
A benefit of cell culture is that cultured cells often display properties that..
What is reflect their origin
This cell signaling pathway is most likely "ON" within immortalized cell lines
What is Ras/MAPK
Information we need to know in advance of an in situ hybridization experiment
What is sequence of target mRNA
Expressed under a cell type-specific promoter, I drive a recombination event that activates GFP expression in specific cells and their progeny
What is Cre recombinase
The purpose of adult (somatic) stem cells is to:
What is maintain tissues
A strategy to hijack cell cycle control and establish an immortal cell line
What is viral transformation
You have a mutant cell line where the STAT nuclear localization signal is constantly exposed. What happens to JAK/STAT signaling in this cell line?
What is limited transcriptional activation
You want to use immunofluorescence to determine if a specific cytokine signaling pathway is on in your tissue. You may choose an antibody targeting this protein
What is phospho-STAT (or phospho-JAK)
You want to study function of a specific cell type; you have identified a marker gene, but don't know which genes are responsible for cell development. You choose this approach
What is cell ablation
Oct4, Sox2 and Klf4 are transcription factors used to generate these
What are induced pluripotent stem cells
Unlike 2D cultured cells, 3D cultured cells need this
What is extracellular matrix
If I express Notch and my neighbour expresses Delta, I will experience this
What is lateral inhibition
You perform FACS on dissected tissue where Cell A express RFP and Cell B expresses GFP. You sort cells based on fluorescence. Draw a representative scatter plot based on expected fluorescence of these cell populations, and identify cells A and B.
RFP and GFP should be represented on separate axes. A population of Cell A will have high RFP signal and low GFP signal, while Cell B will have low RFP signal and high GFP signal.