pGreen Lab
The horizontal gene transfer used in this lab (one of three discussed in lecture).
What is transformation?
PCR unabbreviated.
What is polymerase chain reaction?
If an antibiotic is effective against a certain bacteria, that bacteria is referred to as this term.
What is sensitive?
ELISA unabbreviated.
What is Enzyme-Linked ImmunoSorbent Assay?
EMB is selective for these microbes.
What are gram negative bacteria?
Ampicillin targets this part of bacteria in its mechanism of action.
What is a cell wall?
This prokaryote provides a crucial enzyme that makes PCR possible.
What is Thermus aquaticus?
If an antibiotic is ineffective against a certain bacteria, that bacteria is referred to as this term.
What is resistant?
This type of ELISA test looks for antigens.
What is direct ELISA?
Mannitol salt is differential for this property.
What is fermentation of mannitol?
The recipient in transformation.
What is a live, competent cell?
The specific DNA polymerase used in PCR.
What is Taq polymerase?
This class of drugs targets folic acid synthesis in bacteria.
What are sulfonamides?
This type of ELISA looks for antibodies that a patient has already produced in response to an antigen.
What is indirect ELISA?
The IMViC test is used to tell these types of bacteria apart, commonly known as enterics.
What are gram negative bacteria?
The donor in transformation.
What is a free DNA fragment?
The "starting point" that DNA polymerase binds to before it begins copying.
What is a primer?
The class of drugs that targets the cytoplasmic or cell membrane.
What are polymyxins?
50ml written as µl in scientific notation.
What is 5.0 x 10^4 µl?
These types of microbes commonly test positive on an oxidase test.
What are strict aerobes?
Colonies that were not transformed, but were still able to grow on the ampicillin plate thanks to the resistance from the transformed E. coli.
The nucleic acid and strand of the Covid virus used in this experiment.
What is single stranded RNA?
The mechanism of action for tetracyclines.
What is protein synthesis inhibition?
0.650L converted to ml.
What is 650ml?
The sac-like structure found in rhizopus that contains its asexual spores.
What is a sporangium?