Mutations I
Prokaryotes cannot perform transcription and translation simultaneously. True or False?
False
Mutagens can be carcinogenic. True or false?
True
In Replication the (pick the right answer: DNA or RNA) polymerase copies a new DNA strand from a DNA template strand.
DNA
____ unwinds the double helix of the DNA
Helicase
Restriction enzymes are bacterial enzymes that do not cut DNA at specific base sequences. True or False?
False
What is the difference between free and rough Ribosomes?
Free = cytoplasm
Rough = Endoplasmic Reticulum (ER)
Which of the two categories fall under frameshift mution. Insertion or Deletion?
both lol
According to the Meselson and Stahl experiment demonstrated that the DNA replication is _________
Semiconservative
What keeps the DNA single strands apart or unwound?
Single-Strand Binding Protein (SSBP)
_____ are the "short" circles of DNA
Bacterial Plasmids
The Signal Recognition Particle (SRP) binds to ______
Signal Peptide
_____ are agents that can cause mutation.
Mutagens
DNA Polymerase is an enzyme involved in DNA replication that adds DNA bases only in what direction?
5' --> 3' direction
This enzyme adds a new RNA primer base paired to DNA template
The __________ DNA fragments from 2 or more individuals "cut & pasted" together
Recombinant DNA
What are all of the three tRNA binding sites?
E, P, A binding sites
Which mutation creates a stop codon and prematurely stops translation of polypeptide?
Nonsense Mutation
What is an enzyme that creates an RNA primer base-paired onto DNA template?
Primase
What is the COMPLEMENTARY DNA STRAND of this sequence?
5'-ATG ACG GAT CAGC-'3
3'- TAC TGC CTA GTCG-'5
_________ are the colonies of cells containing various cloned DNA Fragments.
DNA Libraries
What are the three categories of mutations?
Substitution, Insertion, Deletion
What are all the mutations under Substitution?
Silent, Missense, and Nonsense Mutations
Explain why DNA is semi-conservative?
Because each parental DNA strand becomes a template for a new strand.
1. The Primase adds a new (RNA primer or DNA primer) base paired to DNA template
2. The (DNA Pol III or Primase) adds bases onto 3' end of RNA primer to start new Okazaki Fragments.
3. DNA polymersase III adds bases to (finish or start) a new DNA fragment
4. DNA pol. 1 removes old RNA primer and replaces it with (DNA nucleotides or DNA Pol.)
5. DNA (Ligase or Helicase) joins new DNA fragment to growing DNA copy
All the answers are the first choice
The _________ is a cycled reaction using heat-tolerant form of DNA polymerase.
Polymerase Chain Reaction (PCR)