Lecture 1-4 (protein structure, function, purification, characterization)
CH 1: The character of Serine's R-group
What is polar hydroxyl group?
CH 5: These scientists used bacteriophage with radioactive sulfur and phosphorus to prove that DNA is the genetic material.
Who are Hershey and Chase?
CH 11: Remnants of RNA-based viruses in our genome.
What are Retroviral-like Retrotransposons?
CH 14: The sequence similar to the newly-transcribed mRNA, with the exception of Uracil for Thymine.
What is the DNA anti-template/coding/sense strand?
CH 18: This motif in regulatory proteins interacts with the DNA minor groove.
What is Hinge Helix motif
CH 2: Strategy such that a spontaneous reaction may be made 'drive' an nonspontaneous one.
What are Coupled Reactions?
CH 6: Genes evolved from a common ancestor that have been separated by speciation, yet still maintaining similar functions.
What are orthologs?
CH 12: This method detects and quantifies a specific DNA sequence.
CH 15: This protein is involved in assembling the pre-initiation protein complex in eukaryotic transcription.
What is the Mediator protein?
CH 19: Changes to the mRNA structure, such as Stem loops, which occur depending on levels of gene products to regulate the continuation/termination of transcription.
What are Riboswitches?
CH 4: Purpose of ELISA
What is semi-quantitatively identify how much of a protein is present?
CH 8: ssDNA-dependent RNA Polymerase, involved in first synthetic step of DNA Replication
What is Primase?
CH 13: A method to understanding gene function by manipulating a DNA sequence and studying the resulting phenotype/function changes.
What is Reverse Genetics?
CH 17: The number of GTP required to terminate translation.
What is one?
CH 20: The process by which only one copy of a gene in an individual (either from mother or father) is expressed, while the other copy is suppressed.
What is Genetic Imprinting?
CH 3: Acetylation of Lysine impacts amino acid in this manner
What is blocks positive charge?
CH 7: These proteins restrict assembly of nucleosomes into higher order structures, keeping them 'open', by limiting SMC proteins.
What are barrier proteins?
CH 10: The type of DNA repair that fixes pyrimidine crosslinking, commonly thymine dimers.
What is Nucleotide Excision Repair?
CH 16: The enzyme that links amino acids to tRNA during translation.
What is amino acyl-tRNA synthetase?
This type of small non-coding RNA can inhibit translation or impact mRNA stability, leading to degradation
What is miRNA?
CH 9: The dimers that dissociate during (eukaryotic) histone replication.
What are H2A-H2B dimers?