What is results with evidence and explanation, possible errors, and practical applications?
100
These are the four bases found in DNA and how they pair.
What is adenine pairs with thymine and guanine pairs with cytosine?
100
This is the equation to solve dilution problems
What is C1V1 = C2V2?
100
This is one example of a product from the agricultural industry.
What is genetically modified crops? (or something else deemed acceptable)
100
This is the name of the plasmid and the gene we've been genetically manipulating
What is pGLO (plasmid) and GFP (gene)?
200
This is the definition of a constant.
What is a variable that you keep the same throughout entire experiment?
200
These are the locations of transcription and translation in a eukaryotic cell.
What is transcription in the nucleus and translation in the cytoplasm/ribosomes?
200
This is the unit of % (w/v).
What is g/mL?
200
These are the four areas of Biotechnology
What is Medical/Pharmaceutical, Agricultural, Diagnostic, and Industrial/Environmental?
200
These are the two types of restriction enzymes.
What are sticky and blunt?
300
These are three things required for a valid experiment.
What are controls, one variable, a testable hypothesis, etc.
300
These are the three main types of mutations
What are missense, nonsense, and frameshift?
300
There are this many uL in 2.63 L.
What is 2,630,000?
300
This is the type of funding that Universities and non-profit organization usually receive.
What is grants or government money?
300
This is the definition of a vector.
What is something that allows transportation of DNA from one organism to another?
400
These are two things that must be included in a hypothesis.
What are what is being tested and how it will be tested?
400
These are the three modifications made to mRNA before translation.
What are 5' cap, poly A tail, and splicing out introns?
400
This is how many uL of a 30x solution you would add to make 150 mL of a 2x solution.
What is 10,000 uL?
400
This is the general purpose of a Biotechnology company.
What is to make a profit on their product?
400
This is the mRNA sequence and amino acid sequence that corresponds to the DNA sequence: TATGGCTACGCC
What is AUACCGAUGCGG and Ile-Pro-Met-Arg?
500
This is the definition of a positive and negative control.
Positive- something you know the positive outcome before you start (it has been positive in the past)
Negative- a sample that is lacking the actual thing being tested (ex. lacking DNA when genotyping)
500
This is a brief explanation of the central dogma.
What is DNA gets transcribed into mRNA which gets translated into proteins through the use of tRNA.
500
If you added 300 uL of a 75% solution to a final volume of 50 mL, this would be your resulting concentration
What is 0.45%?
500
These are five examples of Biotechnology careers
What is CSI, lab tech, pharmaceutical rep, physician, pharmacist, etc?
500
These are the four types of plates at the end of the pGLO lab and the purpose of each.
What is LB -pGLO (positive control for growth)
LB/Amp -pGLO (negative control for growth)
LB/Amp +pGLO (growth but no glowing)
LB/Amp/Ara + pGLO (our real result)