Show:
DNA and Mitosis
PCR
Restriction Enzymes
Molecular Cloning
MIX
100
Which part of the DNA carries the genetic information: a. Sugar-phosphate backbone b. Base sequence c. Phosphate
b. Base sequence
100
What are the three basic steps of PCR? a. Denature, anneal, & strand displacement b. Denature, anneal & extension c. Strand displacement, synthesis & release d. Reverse-transcription, anneal & extend
b. Denature, anneal & extension
100
A restriction site: a. Encodes for an enzyme that cut DNA b. An enzyme that restricts the growth bacteria c. A stretch of DNA that is recognized and cleaved(cut) by a restriction enzyme d. A random sequence
c. A stretch of DNA that is recognized and cleaved(cut) by a restriction enzyme
100
A plasmid is: A. A clone B. A cell C. A bacterium D. A circular piece of DNA
D. A circular piece of DNA
100
The concentration of your RNA is 250 ng/ul. How many ul of RNA you need to take so to have 1ug of RNA in your reaction?
4ul
200
What is the correct base pairing: a. Purin-purin b. Purin-pyrimidin c. C-G
c. C-G
200
5. You want to detect the presence of the fusion mRNA BCR-ABL. What technique would you use: a. qPCR to quantify and compare the expression of BCR-ABL gene in two different leukemic cell lines b. Conventional PCR to amplify a piece of the BCR-ABL gene c. RT-PCR to detect a piece of the fusion transcript d. qPCR to detect the methylation status of the BCR-ABL promoter
c. RT-PCR to detect the a piece of the fusion transcript
200
Nuclease: a. Catalyzes the disruption of the phosphodiester bond connecting two adjacent nucleic acids b. Catalyzes the disruption of the H bond connecting two complementary nucleotides c. Catalyzes the disruption of the H bond connecting two adjacent nucleotides d. Catalyzes the disruption of the phosphodiester bond connecting two complementary nucleotides
a. Catalyzes the disruption of the phosphodiester bond connecting two adjacent nucleic acids
200
In the plasmid, the multiple cloning site (MCS) is present within the sequence of gene A. Which of the following is true: A. A gene A is always produced because the plasmid contains an ori C B. A gene A is not produced when foreign DNA is successfully inserted into the vector C. A gene A is produced only if foreign DNA is inserted into the vector
B. A gene A is not produced when foreign DNA is successfully inserted into the vector hat is
200
DNA is soluble in ......
water
300
In DNA, complementary nucleotides are joined together by what? a. Covalent bonds b. Hydrogen bonds c. Electrostatic forces
b. Hydrogen bonds
300
In RT-PCR, RNA is copied into complementary DNA (cDNA) by: a. Reverse transcriptase b. Taq DNA polymerase c. RNA polymerase II d. Methyltransferase
a. Reverse transcriptase
300
In what part of the vector the insert DNA is introduced to create a recombinant DNA molecule? a. Promotor b. Shine-Dalgarno c. Randomly d. Multiple Cloning Site (MCS)
d. Multiple Cloning Site (MCS)
300
Recombinant vectors in a genomic library can contain more than one gene. Recombinant vectors in a cDNA library contain only one gene. How this can be explained: A. The host cannot be transformed with a vector carrying more than 1 gene B. Every cDNA fragment carries only one gene C. The 2 libraries are made using different vectors D. cDNA and gDNA are cut with different restriction enzymes
B. Every cDNA fragment carries only one gene
300
The cycle threshold (Ct) is: a. The total number of cycles performed during a real-time PCR reaction b. The number of cycles required for the fluorescent signal to cross the threshold c. The cycle number that a sample enters the plateau phase of PCR d. None of the above
b. The number of cycles required for the fluorescent signal to cross the threshold
400
What cellular process would you expect to immediately disrupt if you added a drug that distroys the mitotis spindle? a. Mitosis b. Protein production c. Transcription d. Fat metabolosm
a. Mitosis
400
In your qPCR reactions, the Ct values for the transcript of your gene of interest and the housekeeping gene are 28 and 15, respectively. What information can you draw by comparing these two values: a. The housekeeping gene is expressed at higher level b. Your gene of interest is expressed at higher level c. The qPCR run is almost over d. The qPCR run has reached the plateau phase
a. The housekeeping gene is expressed at higher level
400
8) Which of the following methods is used to screen colonies to identify the gene of interest: A. Antibiotic selection B. Transformation C. Functional complementation D. Transfection
C. Functional complementation
400
Recombinant DNA (rDNA): a. Naturally found in the nucleus b. Naturally found in mithochondia c. Naturally found in bacteria d. Artificially made in the lab
d. Artificially made in the lab
400
What is the complementary base sequence of the following sequence ATGCCTACT: a. TCATCCGTA b. TACGGATGA c. AGTAGGCAT
b. TACGGATGA
500
At the end of mitosis, one cell has become how many? a. 2 genetically different daughter cells b. 2 genetically identical daughter cells c. 4 genetically different daughter cells d. 4 genetically identical daughter cells
b. 2 genetically identical daughter cells
500
Quantification of real time PCR (qPCR) data using the delta delta Ct relative quantification method: a. Requires the normalization against a reference gene b. Requires a standard curve c. Requires an accurate quantification of the starting material d. Requires that target gene and the reference gene both amplify at low efficiencies
a. Requires the normalization against a reference gene
500
Recombinant technology is possible because: A. The genetic code is universal B. The genetic code is redundant C. Of the existence of bacterial plasmids D. Proteins can’t be purified
A. The genetic code is universal
500
You have created a library and now you want to screen for the Arginine synthesis gene using the functional complementation of a mutant phenotype. Read the following options (a-f) and choose the way to proceed: a. Isolate your DNA fragment from WT cells b. Isolate your DNA fragment from Arginine- mutant cells c. Transform WT cells d. Transform Arginine- mutant cells e. Plate your colonies on minimum media +Arginine f. Plate your colonies on minimum media –Arginine Choose the correct answer: A. a, d, e B. b, c, f C. b, c, e D. a, c, e E. a, d, f
E. a, d, f
500
In a chromosome, sister chromatids are joined together by what? a. Centromere b. Centriole c. Chromosome d. Cytoplasm
a. Centromere