Biotechnology

A

100

The direct manipulation of genes for practical purposes

Genetic engineering 

200

fill in the blank

to make a recombinant dna plasmid ______ is used

restriction enzymes

300

Which of the following is not an application of Biotechnology?

Agriculture 

medicine 

expressing genes

Expressing genes

400

A technique where one strand of a DNA fragment is used as a template for synthesis of nested set of complementary fragments

dideoxy ribonucleotide chain termination sequencing 

500

Gene cloning is useful for two basic purposes:

-to amplify a particular gene.

-to produce a protein product from it.

B

100

Better for attachment

Sticky end

200

used to seal the bonds between restriction fragments

dna ligase

300

We cut the gene of interest by:

Restriction enzymes and Gel Electrophoresis 

400

A process to determine complete nucleotide sequence of a DNA molecule 

DNA sequencing

500

 A single-stranded piece of DNA labeled either with a radioisotope or with non- radioactive label, the nucleotide sequence which is complementary to the target DNA.

Probe

C

100

T or F 

we insert plasmid into DNA

False

200

has multiple pores, a negative and a positive side, uses UV light

Gel Electrophoresis 

300

restriction enzymes cut dna molecules at a dna sequence 

restriction sites

400

what is a gene of interest 

gene chosen to work with

500

A very small pore in a membrane that the single strand DNA molecule is moved through

nanopore 

D

100

small circular DNA molecules that are replicated separately 

Plasmids

200

method used to make many copies of dna

polymerase chain reaction (PCR)

300

plasmid used to clone a foreign gene

cloning vector

400

When using the gel electrophoresis method, the ________ travel faster.

shorter 

500

The DNA of a bacterial cell is protected from the cell's own restriction enzymes by the addition of........

methyl groups

E

100

DNA is slightly...

Negative 

200

seperating the dna fragments based on size using electric current

gel electrophoresis 

300

what is a recombinant DNA molecule? 

A molecule containing DNA from two different sources (species)

400

In PCR what would happen if a standard DNA Pol was used?

it would be denatured along with the DNA during the heating step and we would have to replace it after each step.

500

A scientist was using gel electrophoresis to separate and visualize the fragments in 2 different boxes where both of them were left for 1 hour. He realized that in box A none of the fragments moved upward, while in box B more than 75% of the fragments travelled upward. We can conclude?

Fragments in box A are long so they are slower while in box B they are shorter and travel faster.