Bacterial Transformation
This bacteria was transformed
E. Coli
The charge located near the wells
Negative
Purpose of PCR
Makes lots of copies
If someone has blue eyes, their genotype
bb
How do you say "x2"?
say it right!
This is the name of the plasmid that was introduced into the bacteria
pGlo
This causes the DNA to move
Electricity
What PCR stands for
Polymerase Chain Reaction
If someone has brown eyes, their possible genotypes
Bb, bb
How do you calculate degrees of freedom?
# of phenotypes - 1
This was used to help make the bacteria competent and accept the plasmid
Heat shock and CaCl2
The smallest pieces of DNA move here
End of the gel/furthest
The enzyme used to make copies during PCR
Taq Polymerase
In order for a woman to be colorblind, her genotype
XcXc
The critical value when the df is 1
3.84
This would be seen in a petri dish containing transformed bacteria, arabinose and ampicillin
Glowing colonies
This is used to cut the DNA into pieces
Restriction Enzymes
What is needed to mark the areas to be copied
Primers
Epistatic Cross. When a black lab is crossed with a chocolate lab, some of the pups ended up being yellow. What percent might be yellow?
4 out of 16 or 25%
The X2 is 3.79 for a monohybrid cross resulting in two phenotypes. Can this be accepted?
Yes. The critical value is 3.84
This is the selective marker used for transformation
Antibiotic/Ampicillin
One way in which gel electrophoresis is used for science
Paternity Test/Forensics
The type of DNA being copied in our Cold Spring Harbor DNA Barcoding project
mitochondrial DNA
When two roan cows are crossed, the percentage of cows that will still be roan.
50%
Perform a chi-square for the following:
Observed: 20 Right handed. Expected 15.
Observed: 3 Left handed. Expected 8
4.795