Lectins/Neutralizing Agents
Which lectin is used to identify Bombay phenotype patients?
Ulex europeaus
Reagent cell 2 on an antibody screen is always which Rh haplotype?
R2R2
This blood product modification decreases the frequency of febrile reactions.
Leukoreduction
How much should one unit of RBCs increase a patient's hemoglobin level?
1 g/dL
A platelet transfusion is expected to increase a patient's count by how much?
20-50K
Fresh frozen plasma must be frozen within how many hours?
8 hours
Which ABO type will react with Dolichos biflorus?
A1
Daratumumab interferes with Blood Bank testing how?
By binding to the CD38 antigens on reagent cells causing panreactivity
Which antibody is the most common culprit in a delayed hemolytic transfusion reaction?
Kidd antibodies (Jka and Jkb)
What is the proper dose of plasma?
10-20 ml/Kg
This blood product modification decreases the frequency of HLA alloimmunization.
Leukoreduction
Average amount of iron in a RBC unit
200 mg
This lectin binds to the B antigen
Bandeiraea simplicifolia
A panreactive panel (3-4+ agglutination) with a negative autocontrol is an indication of what type of antibody?
High Frequency
TRALI is most commonly caused by what?
HLA antibodies in the donor unit
Thawed cryo has a shelf-life of how long?
6 hours
4 hours if pooled on site and sterility has been broken
A appropriate 1 hour post-transfusion count but poor 24 hour count is indicative of what?
Non-immune refractoriness
Bleeding, sepsis, consumption
Maximum length of storage of an RBC unit after irradiation
28 days
This antibody that causes mixed field agglutination resembling refractile copper pennies can be neutralized with guinea pig urine
Anti-Sd(a)
Anti-M antibodies most commonly react at what phase of testing?
IS and RT (immediate spin and room temperature)
The most common cause of an anaphylactic transfusion reaction is due to
IgA deficiency and anti-IgA antibodies
What is the threshold for platelet transfusion if the patient is going for surgery?
50 k/uL
What value of a CCI indicates a good response?
>7500
Minimum amount of platelets in an apheresis platelet product
3 x 1011
Which substance can neutralize anti-I?
Breast milk
A mixture or different strengths of reactivity on a panel is an indication of what?
Multiple antibodies and/or the dosage effect
Post-transfusion purpura is most commonly associated with which antibodies?
HPA-1a and HPA-3a
What is the most effective way to reverse warfarin?
Oral Vitamin K if INR is moderately elevated
PCC if the INR is significantly elevated
Do not use plasma
What are the variables in calculating a corrected count increment (CCI)?
Platelet increment
Body Surface Area
Number of platelets transfused
Minimum amount of Factor VIII in a cryo unit
80 IU