Show:
Responses to cellular communication
Differences between the
2D and 3D microenvironment
ECM
Types of Cell Communication
For Fun & more!
100
What method can be used to analyze soluble factors?
Magpix, ELISA, or mass spectrometry
100
Draw the difference between 2D and 3D cell culture.
Go to the board! :)
100
What is the general extracellular matrix composition?
Non-cellular component- proteins, sugars
100
Type of local signaling involved with neurotransmitters?
Synaptic
100
What's your TA's and today's presenters name? :)
Megan, Jordan & María Karina & Molly
200
What is the gold standard method used to evaluate gene expression?
RT-PCR and RT-qPCR (to obtain quantification). RT-PCR is often confused with RT-qPCR by students and scientists because different sources of literature often called them in the same way.
200
What is a cell characteristic that can change between 2D and 3D culture?
Cell migration, morphology, media/drug penetration, cell cycle, cell differentiation, cell-cell interactions, etc.
200
Provide an example of a natural and synthetic ECM that can be used for in vitro experiments.
Natural- Collagen, fibrin, gelatin, HA, Alginate Synthetic- PEG,PLLA
200
Type of signaling when a cell communicates through direct contact with neighbor?
Juxtacrine
200
Cells must constantly maintain their ____ in order to function properly. (Maria's lecture- Cell culture section)
pH
300
In response to a cell signal, what is one example of a molecular level response?
Gene expression, protein production
300
How does 2D vs 3D can affect drugs and nutrients reach to cells?
Drugs and nutrients may not be able to penetrate and reach cells tightly connected to each other.
300
Is collagen a natural or synthetic matrix material?
Natural
300
Type of signaling involved with hormones? Provide an example
Endocrine
300
What is the tractive force produced by a moving viscous fluid on a solid body constraining its motion?
Fluid shear stress
400
In response to a cell signal, what is one example of a cellular level response?
Apoptosis, proliferation, differentiation, invasion
400
How does cell migration changes between 2D and 3D?
Cell migration mechanisms vary dramatically from 2D to 3D. Cells migrate faster in 2D, using different mechanisms than in 3D. The latter requires crawling and adhering sequentially to ECM fibers.
400
What is the enzyme that allows for extracellular matrix remodeling and degradation?
MMPs
400
Type of signaling when a cell communicates through secreting factors to a neighbor? Provide an example
Paracrine
400
What is the name of the fundamental process in which cells migrate directionally when they are exposed to external chemical gradients?
Chemotaxis
500
In response to a cell signal, what is one example of a tissue level response?
Tissue inflammation, ductal hyperplasia, collagen remodeling
500
What is a physical characteristics that is different between 2D and 3D cultures?
In 2D, adhesions are restricted to the x-y plane. In 3D, adhesions are in all 3 dimensions. Stiffness is higher in 2D platforms.
500
What are the two main “clues” that the extracellular matrix provide to the cells?
Biochemical and Biophysical clues
500
Type of signaling when a cell communicates with itself? Provide an example
Autocrine
500
What is Dave's favorite method of transportation?
Bikes & Motorcycles... 2 wheels!