APP Processimg
The full length precursor protein of Amyloid-B
What is APP?
Discovered AD
Who is Dr. Alzheimer?
Name of cell culture-specific hood type
What is biosafety cabinet?
Formula used to calculate stock volume needed for antibody dilutions
What is C1V1=C2V2
Teams qualified for the world cup finals.
What are Argentina and Spain?
Synthesized when b-CTF is cleaved by y-secretase.
What is Amyloid-b?
2 Types of AD
What are sporadic and familial AD?
Assay used to test cell viability
MTT assay
Process used to separate proteins/DNA by size
What is gel electrophoresis?
Disease studied by Liz before she studied AD
What is CF?
Enzyme responsible for amyloid-B degradation
What it ECE?
2 main AD pathologies (causes) in the brain
What are Ab plaques and Tau tangles?
Term used to describe splitting cells into 2 vials once they have reached confluence
What is passage?
Step needed to prevent non-specific binding in ELISA and WB.
What is blocking?
Dye used to stain plaques when performing IF
What is Congo Red?
Enzyme that cleaves APP to form b-CTF
What is b-secretase?
Aggregates that form on the extracellular part of neurons during AD
What are Ab plaques?
Name of the device used to count cells
What is hemocytometer?
C-term APP - Rabbit
What is goat anti-rabbit (or donkey anti-rabbit)
The amount of stock antibody needed to make a 1:3000 dilution of ab, in 15ml final volume?
What is 5uL?
Enzyme that cleaves APP to form a-CTF
What is a-secretase?
Current treatment of AD
What is helping Ab plaques breakdown?
Compound/protein used to detach cells from flask prior to seeding
What is trypsin?
Part of the detection antibody which reacts with TMB substrate to create color during an ELISA.
What is HRP-conjugated antibody?
The wavelength used to detect blue or yellow color on spectrophotometer (absorbance).
What is 650 (blue) and 450 (yellow)?