Clustered & Chained
GNR Playbook
Bent but Not Broken
Acid Fast Files
No Oxygen, No Problem
Toxins & Traits
Round & Reactive
Rod Recon
Miscellaneous Orgs
100

Alpha-hemolytic organism is optochin sensitive and bile soluble.

What is Streptococcus pneumoniae?

100

This test is positive for all members of the Proteeae tribe. (Must say the whole name of the test)

What is phenyalanine deaminase?

100

Fecal cultures are inoculated on thiosulfate-citrate-bile salts-sucrose agar specifically for the isolation of: 

What is Vibrio spp?

100

A skin ulcer organism grows best at 30–32°C and produces toxin-mediated necrosis.

What is Mycobacterium ulcerans?

100

This disk test is used to test for Peptostreptococcus anerobius?

What is SPS?

100

Produces terminal spores and causes muscle spasms.

What is Clostridium tetani?

100

Describe the cellular morphology of Neisseria spp?

What is GNDC, kidney bean shaped?

100

The pulmonary form of anthax is known as: 

What is Woolsorter's Disease?

100

The etiologic agent of whooping cough:

What is Bordetella pertussis?

200

A neonatal sepsis case yields a beta-hemolytic, catalase-negative organism that is CAMP positive

What is Streptococcus agalactiae?

200

Swimmer's ear, a form of external otitis, is commonly caused by this gram-negative organism.

What is Pseudomona aeruginosa?

200

On gram stain, a morphology that resembles "seagull wings" is most characteristic of: 

What is Campylobacter jejuni?

200

The etiological agent for Hansen disease:

What is M. leprae?

200

This organism is an anaerobic GNR that is commonly associated with bacterial vaginosis: 

What is Mobiluncus spp?

200

Egg yolk agar showing precipitate in the medium surround the colony is positive for:

What is lecithinase?

200

This organism exhibits a hockey-puck appearance when pushed across the agar.

What is Moraxella catarrhalis?

200

Motile, beta-hemolytic organism causing food poisoning.

What is Bacillus cereus?

200

This organism is found in the mouth and human respiratory tract of humans. When grown on BAP, it pits into the agar and creates a bleach-like odor?

What is Eikenella corrodens?

300

Exfoliatin produced by S. aureus is responsible for causing:

What is Scalded Skin Syndrome?

300

Hemolytic uremic syndrome is a complication after infection with: 

What is E. coli O157:H7?

300

The symptom of diffuse, watery diarrhea that produces a relatively clear stool containing mucus flecks is suggestive of: 

What is Vibrio chlorae?

300

A slow grower produces pigment only after light exposure.

What is a photochromogen?

300

Black-pigmented anaerobe associated with oral infections.

What is Prevotella melaninogenica?


300

This organism causes pseudomembranous colitis after antibiotics.

What is Clostridioides difficile?


300

CSF shows intracellular diplococci that ferment glucose and maltose.

What is Neisseria meningitidis?

300

This organism is associated with infections in humans often linked to deli meats and improperly pasteurized dairy products.

What is Listeria monocytogenes?

300

What is hallmark sign of an anaerobic infection?

What is a Foul odor?

400

Blood cultures grow alpha-hemolytic streptococci that are optochin resistant and associated with endocarditis.


What are viridans streptococci?

400

Cefsulodin irgasan novobiocin agar is recommended for this organism:

What is Yersinia enterocolitica?

400

A halophilic organism from seawater wound infection causes rapid necrosis.


What is Vibrio vulnificus?

400

This mycobacterium appears buff colored after exposure to light and is niacin positive.

What is M. tuberculosis?

400

This anaerobic gram-negative rod produces dark pigmented colonies and may demonstrate a brick-red fluorescence under UV light, commonly associated with oral infections.

What is Porphyromonas spp?

400

This bacterium is potentially lethal causing food poisoning often associated with improperly canned food.

What is C. botulinum?

400

This organism oxidizes glucose, maltose, and lactose/

What is N. lactamica?

400

Palisading rods producing toxin-mediated pseudomembrane.

What is Corynebacterium diphtheriae?

400
This microorganism will grow only on culture media supplemented with either cysteine or cystine?
What is Francisella tularensis?
500

A catalase negative gram positive cocci is isolated from a urine sample of a hospitalized patient. The bacterium produced a black pigment on bile esculin agar and formed acid from glucose in the presence of 6.5% NaCl. The most likely identification of this bacterium is: 

What is Enterococcus spp.?

500

A nonlactose fermenter is isolated from a stool specimen. A biochemical assay identifies the isolate as Shigella spp. The isolate demonstrates agglutination in Shigella group B antisera. The Shigella species is: 

What is Shigella flexneri?

500

Infection of the gastic mucosa leading to gastris and peptic ulcers is associated with: 

What is Helicobacter pylori?

500

Which of the following specimes would be routine decontaminated when trying to recover Mycobacterium spp?

A. Sputum

B. Pleural fluid

C. Lung biopsy

D. CSF

A. Sputum

500
This is an obligate anerobic, GNR, recovered from an abdominal wound, that is resistant to penicillin. Growth of this organism was not inhibited by 20% bile. 

What is Bacteroides fragilis?

500

This organism is part of the vaginal normal flora that helps resist the onset of bacterial vaginosis?

What is Lactobacillus spp?

500

Erythromycin eye drops are routinely administered to infants to prevent infections caused by: 

What is Neisseria gonorrhoeae?

500

Partially acid-fast branching rods causing pulmonary disease.

What is Nocardia?

500

This organism is a gram-negative rod that produces infections most often acquired from environmental sources, such as contaminated AC units.

What is Legionella pneumophila?

600

A microbiology tech receives in a throat swab. While streaking the blood agar plate, she stabs the agar. After incubation, she notices hemolysis around the bottom of the stab line and on top. Explain why she stabbed the agar.

When culturing a throat swab, one of the pathogens you look for is S. pyogenes. This organism produced Streptolysin S (oxygen stable) and O (oxygen labile). 

600

A patient presents with gastroenteritis after handling freshwater fish. Stool culture yields non-lactose fermenting colonies on MacConkey agar. The organism is motile, oxidase negative, and produces H2S.

Biochemical results:

  • TSI: K/A, H2S positive, no gas
  • Indole: positive
  • Urease: negative
  • Lysine decarboxylase: positive

What is Edwardsiella tarda?

600

A patient presents with watery diarrhea after swimming in freshwater. Stool culture yields non-lactose fermenting colonies on MacConkey agar. The organism is oxidase positive and grows well on routine media without added salt.

Biochemical results:

  • TSI: A/A, no H2S
  • Indole: positive
  • Oxidase: positive
  • Growth in 6% NaCl: negative

What is Aeromonas?

600

An AIDS patient develops disseminated infection from a nonchromogen organism that is Niacin negative, nitrate positive, SQ negative, 68°C variable.

What is Mycobacterium avium complex?

600

This is an anaerobic, long, thin, gram-negative rod with pointed ends. It is inhibited by bile salt and sensitive to kanamycin.

What is Fusobacterium?

600

Chronic suppurative infection producing granulomatous lesions with a molar tooth-like appearance on agar and branching rods under a microscope.

What is Actinomyces israelii?

600

Name three media plates used specifically for the cultivation of Neisseria spp?

What is Chocolate agar, MTM/TM, and NYC agar?

600

Zoonotic infection with well-demarcated skin lesion after animal exposure.

What is Erysipelothrix rhusiopathiae?

600

The sexually acquired disease characterized by genital ulcers and tender inguinal lymphadenopathy, which is caused by a small gram-negative bacillus known as: 

What is Chancroid?

700

How would you differentiate Leuconostoc and Pediococcus?

Leuconostoc is Vancomycin resistant; Pediococcus is sens

700

A 10-year-old patient presents with right lower quadrant abdominal pain, fever, and leukocytosis, initially suspected to be acute appendicitis. Imaging reveals mesenteric lymphadenitis instead of an inflamed appendix.

Stool culture yields non-lactose fermenting colonies. The organism is a gram-negative coccobacillus demonstrating motility at 25°C but not at 37°C. It grows well after cold enrichment.

Biochemical results:

  • TSI: A/A, no H2S
  • Urease: positive
  • Motility: positive at 25°C only
  • IMViC: --+-

What is Yersinia enterocolitica?

700

A patient presents with acute watery diarrhea and abdominal cramping after consuming raw oysters. Stool culture yields a curved, oxidase-positive gram-negative rod that grows well in high-salt conditions.

On selective media:

  • TCBS agar: green colonies (non-sucrose fermenter)

Biochemical results:

  • TSI: A/A, no H2S
  • Oxidase: positive
  • Growth in 6% NaCl: positive

What is V. parahaemolyticus?

700

This organism is associated with respiratory infections. Its cells are long and broad with distinct crossbanding. It is Niacin negative, nitrate positive, both catalase positive

What is M. kansaii?

700

This is an anaerobic GNC that resides in the oral cavity and is Vancomycin resistant. 

What is Viellonella?

700

Name 3 Naegler positive organisms.

What are C. perfringens, C. sordelli. C. bifermentans, or C. novyi?

700

n older adult with chronic lung disease presents with productive cough and exacerbation of COPD. Sputum Gram stain shows gram-negative diplococci. The organism is oxidase positive and grows well on blood and chocolate agar.

Biochemical results:

  • Glucose fermentation: negative
  • Maltose fermentation: negative
  • DNase: positive
  • Butyrate esterase: positive

What is M. cattarrhalis?

700

This organism causes an allergic respiratory disease called "Farmer's Lung". It is also associated with actinomycotic mycetomas, pustular cellulitis, and hypersensitivity pneumonitis. 

What is Nocardia?

700

An immunocompromised patient presents with rapidly progressive cellulitis and sepsis following a dog bite. Culture yields a thin, spreading gram-negative rod demonstrating gliding motility.

The organism grows best in increased CO₂ (capnophilic conditions) and is part of the normal oral flora of dogs and humans.

Biochemical/phenotypic clues:

  • Oxidase: variable
  • Catalase: variable
  • Growth: enhanced with CO₂
  • Motility: gliding (no flagella)

What is Capnocytophaga canimorus or Capnocytophaga spp?